Rabbit NHERF1 Polyclonal Antibody | anti-SLC9A3R1 antibody

NHERF1 Rabbit pab Antibody

Cat. Num. AAA125205

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity purification
• Synonyms: NHERF1; Polyclonal Antibody; NHERF1 Rabbit pab Antibody; SLC9A3R1; NHERF1 ; NHERF; anti-SLC9A3R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: Liquid
• Sequence Length: 358

• Applicable Applications for anti-SLC9A3R1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Western Blot (WB): 1/2000-5000; Immunohistochemistry (IHC): 1/50; Immunocytochemistry (ICC): 1/50; Immunoprecipitation (IP): 1/20; Flow Cytometry (FC/FACS): 1/50-100
• Immunogen: A Fusion protein of human NHERF1
• Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol,
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SLC9A3R1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Related Product Information for anti-SLC9A3R1 antibody

Rabbit polyclonal to Human NHERF1

Na+/H+ exchanger regulatory factor (NHERF1 or EBP-50) is one of several related PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus (2,3). NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells (2,3). Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer (4). NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking (6).

References

1. Donowitz, M. et al. (2005) J Physiol 567, 3-11. 2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14. 3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62. 4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20. 5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87. 6. Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NM_004252.4
• UniProt Accession #: O14745; O43552; Q86WQ5
• Molecular Weight: 358
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_HUMAN