Testing Data
(THP-1 cells were stained with MBS609683 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Mouse anti-Human Semaphorin 4B Monoclonal Antibody | anti-SEMA4B antibody
Semaphorin 4B (SEMA4B, Semaphorin-4B, KIAA1745, SEMAC, UNQ749/PRO1480)
Gene Names
SEMA4B; SemC; SEMAC; KIAA1745; MGC131831
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Semaphorin 4B; Monoclonal Antibody; Semaphorin 4B (SEMA4B; Semaphorin-4B; KIAA1745; SEMAC; UNQ749/PRO1480); Anti -Semaphorin 4B (SEMA4B; anti-SEMA4B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
10K60
Specificity
Recognizes human Semaphorin 4B.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-SEMA4B antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 0.5-1ug/ml
Dilution: Flow Cytometry: 0.5-1ug/ml
Immunogen
Purified, NS0-derived, recombinant human Semaphorin 4B (aa39-712; Accession #Q9NPR2).
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Semaphorin 4B.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(THP-1 cells were stained with MBS609683 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Testing Data
(THP-1 cells were stained with MBS609683 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Related Product Information for anti-SEMA4B antibody
Semaphorin 4B (Sema4B; also SemaC) is a 95-100kD, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Semaphorin 4B is a type I transmembrane glycoprotein that contains one Sema domain (aa65-502), a PSI region (aa520-574), and an Ig-like C2-type domain (aa599-658) in its extracellular domain. There is one potential soluble splice variant that shows a 29aa substitution for aa707-832. Over aa39-712, human Semaphorin 4B shares 86% aa identity with mouse Semaphorin 4B.
Product Categories/Family for anti-SEMA4B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,750 Da
NCBI Official Full Name
semaphorin-4B
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
NCBI Official Symbol
SEMA4B
NCBI Official Synonym Symbols
SemC; SEMAC; KIAA1745; MGC131831
NCBI Protein Information
semaphorin-4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B
UniProt Protein Name
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 4B
Protein Family
UniProt Gene Name
SEMA4B
UniProt Entry Name
Q2NL81_HUMAN
Uniprot Description
SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 15q25
Cellular Component: plasma membrane; integral to membrane; synapse
Molecular Function: receptor activity
Biological Process: nervous system development; cell differentiation
Research Articles on SEMA4B
Similar Products
Product Notes
The SEMA4B sema4b (Catalog #AAA609683) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Semaphorin 4B (SEMA4B, Semaphorin-4B, KIAA1745, SEMAC, UNQ749/PRO1480) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 0.5-1ug/ml. Researchers should empirically determine the suitability of the SEMA4B sema4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 4B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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