Rabbit SUMO2 Polyclonal Antibody | anti-SUMO2 antibody

Anti-SUMO2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100963

• Gene Names: SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SUMO2; Polyclonal Antibody; Anti-SUMO2 Antibody; Rabbit Polyclonal; SUMO2 Antibody; Rabbit PAb; Antigen Affinity Purified; small ubiquitin-like modifier 2; Anti-HSMT3 Antibody; Anti-Smt3A Antibody; Anti-SMT3B Antibody; Anti-SMT3H2 Antibody; Anti-SUMO3 Antibody; anti-SUMO2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SUMO2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SUMO2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human SUMO2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SUMO2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of SUMO2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human SUMO2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..)

Immunoprecipitation (IP)

(SUMO2 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell Lysate2 uL anti-SUMO2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUMO2 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)

Western Blot (WB)

(Anti-SUMO2 rabbit polyclonal antibody at 1:500 dilutionLane A: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SUMO2 antibody

SUMO2 gene silencing promotes proliferation, and inhibits the apoptosis and senescence of NPCs in rats with IDD through the down-regulation of the p53 signaling pathway. Thus, SUMO2 is a potential target in the treatment of Intervertebral disc degeneration (IDD). The small ubiquitin-related modifier protein (SUMO) functions as a posttranslational modification system and SUMO2/3 subtypes are often activated in early stress. SUMO2 as a novel activator of Cn-NFAT signaling in cardiomyocytes.

Product Categories/Family for anti-SUMO2 antibody

NF-kB (NFkB) Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 54792071
• NCBI GeneID: 6613
• NCBI Accession #: NP_001005849.1
• NCBI GenBank Nucleotide #: NM_001005849.1
• UniProt Accession #: P61956; P55855; Q32Q42; Q6IPZ6; Q96HK1; B2R4I2
• Molecular Weight: 10,871 Da
• NCBI Official Full Name: small ubiquitin-related modifier 2 isoform b
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: SUMO2
• NCBI Official Synonym Symbols: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• NCBI Protein Information: small ubiquitin-related modifier 2; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO2
• UniProt Synonym Gene Names: SMT3A; SMT3H2; SUMO-2; Smt3A
• UniProt Entry Name: SUMO2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; PML body; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: cellular protein metabolic process; protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; post-translational protein modification