Western Blot (WB)
(Western Blot analysis of extracts from 293T, C2C12 and C6 cells using MBS605436.)
Rabbit Ras, pan, N,H,K Monoclonal Antibody | anti-Ras85D antibody
Ras, pan, N,H,K
Gene Names
Ras85D; C-ras1; CG9375; D-Ras; D-ras-1; D-ras1; D-Ras1; Dm Ras1; DmelCG9375; Dmras85D; dRas; Dras; DRas; dras1; dRas1; dRAS1; Dras1; DRAS1; dRas85D; Dras85D; DRas85D/Ras; E(faf); E(sev)3C; EK3-4; fs(3)05703; l(3)06677; l(3)s1747; p21[Ras1]; ras; Ras; RAS; ras 1;
Reactivity
Drosophila, Human, Monkey, Mouse, Rat, Yeast
Applications
ELISA, Western Blot
Purity
SupernatantSupernatant
Synonyms
Ras; pan; N; H; K; Monoclonal Antibody; Anti -Ras; anti-Ras85D antibody
Host
Rabbit
Reactivity
Drosophila, Human, Monkey, Mouse, Rat, Yeast
Clonality
Monoclonal
Isotype
IgG
Clone Number
7H56
Specificity
Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity mouse, monkey, rat, Drosophila, S. cerevisiae.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-Ras85D antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human K-Ras (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of extracts from 293T, C2C12 and C6 cells using MBS605436.)
Western Blot (WB)
(Western Blot analysis of extracts from 293T, C2C12 and C6 cells using MBS605436.)
Related Product Information for anti-Ras85D antibody
The 21kD guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
Product Categories/Family for anti-Ras85D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Ras oncogene at 85D
NCBI Official Synonym Full Names
Ras oncogene at 85D
NCBI Official Symbol
Ras85D
NCBI Official Synonym Symbols
C-ras1; CG9375; D-Ras; D-ras-1; D-ras1; D-Ras1; Dm Ras1; DmelCG9375; Dmras85D; dRas; Dras; DRas; dras1; dRas1; dRAS1; Dras1; DRAS1; dRas85D; Dras85D; DRas85D/Ras; E(faf); E(sev)3C; EK3-4; fs(3)05703; l(3)06677; l(3)s1747; p21[Ras1]; ras; Ras; RAS; ras 1;
NCBI Protein Information
CG9375 gene product from transcript CG9375-RA; CG9375-PA; Ras GTPase; Ras oncogene at 85B; Ras oncogene at 85D; Ras1; Ras85D-PA; enhancer of faf
Protein Family
Research Articles on Ras85D
Similar Products
Product Notes
The Ras85D (Catalog #AAA605436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ras, pan, N,H,K reacts with Drosophila, Human, Monkey, Mouse, Rat, Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Ras, pan, N,H,K can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Ras85D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ras, pan, N,H,K, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
