Rabbit Ras Polyclonal Antibody | anti-Nras antibody

RAS Antibody: Biotin

Cat. Num. AAA806066

• Gene Names: Nras; N-ras; AV095280
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Ras; Polyclonal Antibody; RAS Antibody: Biotin; GTPase Hras; GTPase Kras; GTPase Nras; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH1; anti-Nras antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-Nras antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:1000 (WB)
• Conjugate: Biotin
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction.)

Related Product Information for anti-Nras antibody

Background Info: Detects ~21kDa.

Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

Product Categories/Family for anti-Nras antibody

Cell Signaling

References

1. Boguski M.S. and McCormick F. (1993) Nature 366: 643-654. 2. Avruch J., et al., (1994) Trends Biochem. Sci.19: 279-283. 3. Buday L. and Downward J. (1993) Cell 73: 611-620. 4. Huang D.C., et al., (1993) Mol. Cell Biol. 13: 2420-2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682-4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114- 131.

NCBI and Uniprot Product Information

• NCBI GI #: 111154109
• NCBI GeneID: 18176
• NCBI Accession #: NP_035067.2
• NCBI GenBank Nucleotide #: NM_010937.2
• UniProt Accession #: B2KGV5; P08556
• Molecular Weight: 21,199 Da
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma ras oncogene
• NCBI Official Symbol: Nras
• NCBI Official Synonym Symbols: N-ras; AV095280
• NCBI Protein Information: GTPase NRas; transforming protein N-Ras
• UniProt Protein Name: GTPase NRas
• Protein Family: Ras protein
• UniProt Gene Name: Nras
• UniProt Entry Name: RASN_MOUSE

Uniprot Description

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein, monomeric; G protein

Cellular Component: Golgi apparatus; membrane; plasma membrane; intracellular

Molecular Function: GTPase activity; GTP binding; protein complex binding; nucleotide binding

Biological Process: negative regulation of skeletal muscle development; regulation of cell cycle; small GTPase mediated signal transduction; Ras protein signal transduction; signal transduction