Mouse Hexokinase Type II Monoclonal Antibody | anti-HK2 antibody

Hexokinase Type II (HK2, HKII)

Cat. Num. AAA603433

• Gene Names: HK2; HKII; HXK2; DKFZp686M1669
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Hexokinase Type II; Monoclonal Antibody; Hexokinase Type II (HK2; HKII); Anti -Hexokinase Type II (HK2; anti-HK2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 8G1
• Specificity: Recognizes Hexokinase Type II (HK2, HKII)
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.2, 0.1% sodium azide.

• Applicable Applications for anti-HK2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Recombinant human Hexokinase 2 (1-917aa) purified from E. coli. MW: 102kD
• Hybridoma: Derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase 2 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HK2 antibody

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

Product Categories/Family for anti-HK2 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 15553127
• NCBI GeneID: 3099
• NCBI Accession #: NP_000180.2
• NCBI GenBank Nucleotide #: NM_000189.4
• UniProt Accession #: P52789; Q8WU87; Q9UN82; D6W5J2; Q09LL6; Q53QX9; Q53SG7; Q68E10; Q7Z7Q6
• Molecular Weight: 102,380 Da
• NCBI Official Full Name: hexokinase-2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2; DKFZp686M1669
• NCBI Protein Information: hexokinase-2; HK II; OTTHUMP00000160433; OTTHUMP00000202738; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
• UniProt Protein Name: Hexokinase-2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK2
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Mitochondrial; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; glucose metabolic process; regulation of glucose import; pathogenesis; glucose transport; transmembrane transport