Western Blot (WB)
(Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. )
Mouse alpha-tubulin Monoclonal Antibody | anti-TUBA4A antibody
alpha-tubulin Mouse mAb
IP: 1:200
IF: 1:100-200
IHC: 1:200-500
Western Blot (WB)
(Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. )
Western Blot (WB)
(Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. )
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using alpha tubulin (EM1034)Mouse mAb diluted at 1:500.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using alpha tubulin (EM1034)Mouse mAb diluted at 1:500.)
Testing Data
(1. Input: Mouse Brain Tissue Lysate 2. IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1034 1:5,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000)
Testing Data
(1. Input: Mouse Brain Tissue Lysate 2. IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1034 1:5,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000)
Immunofluorescence (IF)
(IF analysis of Hela with EM1034(Left) and DAPI (Right) diluted at 1:100.)
Immunofluorescence (IF)
(IF analysis of Hela with EM1034(Left) and DAPI (Right) diluted at 1:100.)
NCBI and Uniprot Product Information
NCBI Description
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
Uniprot Description
TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: microtubule; extracellular region; cytosol
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process
Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia
Research Articles on TUBA4A
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Product Notes
The TUBA4A tuba4a (Catalog #AAA8808499) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The alpha-tubulin Mouse mAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:5,000-10,000 IP: 1:200 IF: 1:100-200 IHC: 1:200-500. Researchers should empirically determine the suitability of the TUBA4A tuba4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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