Rabbit anti-Sheep 14-3-3E Polyclonal Antibody | anti-14-3-3epsilon antibody
14-3-3E, NT (YWHAE, 14-3-3 Protein epsilon)
Gene Names
14-3-3epsilon; 14-3-3; 14-3-3 epsilon; 14-3-3-e; 14-3-3-epsilon; 14-3-30epsilon; 14-3-3e; 14-3-3EPSILON; 14-3-3omicron; 1433epsilon; 143E_DROME; anon-WO0172774.141; anon-WO02059370.52; CG31196; CT24092; D14-3-3e; d14-3-3epsilon; D14-3-3epsilon; DmelCG31196; EK3-5; eps;
Reactivity
Sheep
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
SerumSerum
Synonyms
14-3-3E; Polyclonal Antibody; NT (YWHAE; 14-3-3 Protein epsilon); Anti -14-3-3E; anti-14-3-3epsilon antibody
Host
Rabbit
Reactivity
Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes sheep YWHAE. Species sequence homology: Bovine, chicken, human, mouse, rabbit, rat.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Applicable Applications for anti-14-3-3epsilon antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:400
Dilution: Western Blot: 1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:400
Immunogen
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-14-3-3epsilon antibody
14-3-3 epsilon is a member of the 14-3-3 family which consists of 30kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.
Product Categories/Family for anti-14-3-3epsilon antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,799 Da
NCBI Official Full Name
14-3-3epsilon, isoform C
NCBI Official Symbol
14-3-3epsilon
NCBI Official Synonym Symbols
14-3-3; 14-3-3 epsilon; 14-3-3-e; 14-3-3-epsilon; 14-3-30epsilon; 14-3-3e; 14-3-3EPSILON; 14-3-3omicron; 1433epsilon; 143E_DROME; anon-WO0172774.141; anon-WO02059370.52; CG31196; CT24092; D14-3-3e; d14-3-3epsilon; D14-3-3epsilon; DmelCG31196; EK3-5; eps;
NCBI Protein Information
CG31196 gene product from transcript CG31196-RB; 14-3-3ε 14-3-3e; 14-3-3epsilon; 14-3-3epsilon-PA; 14-3-3epsilon-PB; 14-3-3epsilon-PC; 14-3-3epsilon-PD; CG31196-PA; CG31196-PB; CG31196-PC; CG31196-PD; Tyrosine-3-monooxygenase; suppressor of Ras85D 3-9
UniProt Protein Name
14-3-3 protein epsilon
UniProt Gene Name
14-3-3epsilon
UniProt Synonym Gene Names
14-3-3e; SR3-9
UniProt Entry Name
1433E_DROME
Uniprot Description
Function: Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization. Ref.6
Subunit structure: Homodimer
By similarity. Interacts with phosphorylated yki. Ref.5 Ref.6
Sequence similarities: Belongs to the 14-3-3 family.
Research Articles on 14-3-3epsilon
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Product Notes
The 14-3-3epsilon 14-3-3epsilon (Catalog #AAA6008127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3E, NT (YWHAE, 14-3-3 Protein epsilon) reacts with Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3E can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:3000 Immunohistochemistry (Formalin fixed paraffin embedded): 1:400. Researchers should empirically determine the suitability of the 14-3-3epsilon 14-3-3epsilon for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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