Mouse anti-Human 14-3-3, tau Monoclonal Antibody | anti-YWHAQ antibody

14-3-3, tau (14-3-3 Protein theta, 14-3-3 Protein tau, 14-3-3 Protein T-cell, Protein HS1, YWHAQ)

Cat. Num. AAA6008644

• Gene Names: YWHAQ; 1C5; HS1; 14-3-3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: 14-3-3; tau; Monoclonal Antibody; tau (14-3-3 Protein theta; 14-3-3 Protein tau; 14-3-3 Protein T-cell; Protein HS1; YWHAQ); Anti -14-3-3; anti-YWHAQ antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AT1A1
• Specificity: Recognizes human YWHAQ.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-YWHAQ antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 10ug/ml
• Immunogen: Recombinant corresponding to aa1-245 from human 14-3-3 tau expressed in E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with YWHAQ antibody at 10ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Pancreas (formalin-fixed, paraffin-embedded) stained with YWHAQ antibody at 10ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Testing Data

Testing Data

Related Product Information for anti-YWHAQ antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. Recombinant human YWHAQ was expressed in E. coli and purified by using conventional chromatography techniques.

Product Categories/Family for anti-YWHAQ antibody

Antibodies; Abs to 14-3-3

NCBI and Uniprot Product Information

• NCBI GI #: 5803227
• NCBI GeneID: 10971
• NCBI Accession #: NP_006817.1
• NCBI GenBank Nucleotide #: NM_006826.3
• UniProt Accession #: P27348; Q567U5; Q5TZU8; Q9UP48; D6W4Z5
• Molecular Weight: 27,764 Da
• NCBI Official Full Name: 14-3-3 protein theta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• NCBI Official Symbol: YWHAQ
• NCBI Official Synonym Symbols: 1C5; HS1; 14-3-3
• NCBI Protein Information: 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell
• UniProt Protein Name: 14-3-3 protein theta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAQ
• UniProt Entry Name: 1433T_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting