Mouse anti-Human, Mouse Neurofilament 150, 200kD Monoclonal Antibody | anti-NF1 antibody

Neurofilament 150, 200kD

Cat. Num. AAA600613

• Gene Names: NF1; WSS; NFNS; VRNF; FLJ21220; DKFZp686J1293
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified
• Synonyms: Neurofilament 150; 200kD; Monoclonal Antibody; Anti -Neurofilament 150; anti-NF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5G16
• Specificity: Reacts with all three neurofilament proteins, Mr 68kD, 150kD and 200kD molecules in mouse and recognizes proteins 150kD and 200kD in human.
• Purity/Purification: Affinity Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-NF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Western Blot.; Dilution: Western Blot: 1:500
• Immunogen: Purified neurofilament triplet proteins from human spinal cord
• Hybridoma: Derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NF1 antibody

The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.

Product Categories/Family for anti-NF1 antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 292354
• NCBI GeneID: 4763
• UniProt Accession #: P21359; O00662; Q14284; Q14930; Q9UMK3; Q14180; Q14931; Q4W6X4; Q59FX3; Q7Z3J5; Q9UMU3
• Molecular Weight: 319,372 Da
• NCBI Official Full Name: neurofibromin
• NCBI Official Synonym Full Names: neurofibromin 1
• NCBI Official Symbol: NF1
• NCBI Official Synonym Symbols: WSS; NFNS; VRNF; FLJ21220; DKFZp686J1293
• NCBI Protein Information: neurofibromin; OTTHUMP00000163771; OTTHUMP00000163772; OTTHUMP00000166089; neurofibromatosis-related protein NF-1
• UniProt Protein Name: Neurofibromin
• Protein Family: Neurofibromin
• UniProt Gene Name: NF1
• UniProt Entry Name: NF1_HUMAN

NCBI Description

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq]

Uniprot Description

NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Tumor suppressor; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: membrane; axon; cytoplasm; dendrite; nucleolus; nucleus

Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylcholine binding

Biological Process: negative regulation of Rac protein signal transduction; neural tube development; negative regulation of MAP kinase activity; Schwann cell development; extracellular matrix organization and biogenesis; collagen fibril organization; positive regulation of apoptosis; heart development; negative regulation of transcription factor import into nucleus; negative regulation of neuroblast proliferation; regulation of glial cell differentiation; positive regulation of adenylate cyclase activity; negative regulation of neurotransmitter secretion; skeletal muscle development; adrenal gland development; negative regulation of cell-matrix adhesion; forebrain morphogenesis; regulation of synaptic transmission, GABAergic; myelination in the peripheral nervous system; camera-type eye morphogenesis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; negative regulation of endothelial cell proliferation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; cerebral cortex development; actin cytoskeleton organization and biogenesis; forebrain astrocyte development; metanephros development; regulation of long-term neuronal synaptic plasticity; negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; wound healing; regulation of cell-matrix adhesion; regulation of blood vessel endothelial cell migration; smooth muscle development; sympathetic nervous system development; positive regulation of neuron apoptosis; negative regulation of Ras protein signal transduction; visual learning; cell communication; negative regulation of cell migration; regulation of bone resorption; negative regulation of MAPKKK cascade; MAPKKK cascade; liver development; regulation of angiogenesis; peripheral nervous system development; osteoblast differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of angiogenesis; pigmentation; spinal cord development; Ras protein signal transduction; negative regulation of astrocyte differentiation; response to hypoxia; artery morphogenesis; brain development; cognition

Disease: Neurofibromatosis, Familial Spinal; Watson Syndrome; Juvenile Myelomonocytic Leukemia; Neurofibromatosis, Type I; Neurofibromatosis-noonan Syndrome