Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) Standard Curve.)
Human Phosphoinositide 3-Kinase Regulatory Subunit 4 (PIK3R4) ELISA Kit | PIK3R4 elisa kit
Human Phosphoinositide 3-Kinase Regulatory Subunit 4 (PIK3R4) ELISA Kit
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human
Synonyms
Phosphoinositide 3-Kinase Regulatory Subunit 4 (PIK3R4); Human Phosphoinositide 3-Kinase Regulatory Subunit 4 (PIK3R4) ELISA Kit; PIK3R4; MGC102700; VPS15; p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide-3-kinase; reg; PIK3R4 elisa kit
Reactivity
Human
Specificity
Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIK3R4. No significant cross-reactivity or interference between Human PIK3R4 and analogues was observed.
Sequence Length
1358
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) Standard Curve.)
Related Product Information for PIK3R4 elisa kit
Description: This Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R4 in samples. An antibody specific for PIK3R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: In yeast, the vps15 gene product is a 150-kD serine/threonine kinase that forms a complex with a phosphatidylinositol 3-kinase, vps34 (PIK3C3); in human this complex is involved in protein trafficking. By screening a cDNA library, Panaretou et al. (1997) cloned the homolog of the yeast vps15 gene, which they termed p150. The p150 cDNA encodes a predicted polypeptide of 1,358 amino acids. The amino acid sequence is 29.6% identical to that of yeast vps15 protein. Northern blot analysis revealed that the p150 gene is ubiquitously expressed as a 5.5-kb transcript. Panaretou et al. (1997) found that the p150 gene interacts with PIK3C3 in vivo.
Background: In yeast, the vps15 gene product is a 150-kD serine/threonine kinase that forms a complex with a phosphatidylinositol 3-kinase, vps34 (PIK3C3); in human this complex is involved in protein trafficking. By screening a cDNA library, Panaretou et al. (1997) cloned the homolog of the yeast vps15 gene, which they termed p150. The p150 cDNA encodes a predicted polypeptide of 1,358 amino acids. The amino acid sequence is 29.6% identical to that of yeast vps15 protein. Northern blot analysis revealed that the p150 gene is ubiquitously expressed as a 5.5-kb transcript. Panaretou et al. (1997) found that the p150 gene interacts with PIK3C3 in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 4
NCBI Official Symbol
PIK3R4
NCBI Official Synonym Symbols
p150; VPS15
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 4
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
Protein Family
UniProt Gene Name
PIK3R4
UniProt Synonym Gene Names
PI3-kinase regulatory subunit 4
UniProt Entry Name
PI3R4_HUMAN
Uniprot Description
PIK3R4: Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Kinase, lipid; Protein kinase, Other; Other group; VPS15 family
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: membrane; late endosome; nuclear pore; axoneme; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: macroautophagy; phospholipid metabolic process; late endosome to vacuole transport; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; protein targeting to vacuole; toll-like receptor 9 signaling pathway; peroxisome degradation; protein amino acid phosphorylation
Research Articles on PIK3R4
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Product Notes
The Human PIK3R4 pik3r4 (Catalog #AAA9716020) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716020 ELISA Kit recognizes Human PIK3R4. It is sometimes possible for the material contained within the vial of "Phosphoinositide 3-Kinase Regulatory Subunit 4 (PIK3R4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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