Rabbit Neurofibromin / NF1 Polyclonal Antibody | anti-NF1 antibody

Rabbit Polyclonal (IgG) to Human Neurofibromin / NF1

Cat. Num. AAA244810

• Gene Names: NF1; WSS; NFNS; VRNF
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: Neurofibromin / NF1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human Neurofibromin / NF1; Anti-Neurofibromin / NF1 Antibody (aa1551-1600) IHC-plus; NF1; VRNF; Neurofibromin 1; NFNS; WSS; Neurofibromin; Human Neurofibromin; anti-NF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NF1 Antibody detects endogenous levels of total NF1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2818

• Applicable Applications for anti-NF1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (1:100), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NF1 (aa1551-1600).
• Immunogen: Neurofibromin / NF1 antibody was raised against synthetic peptide from human NF1 (aa1551-1600).
• Antigen Modification: aa1551-1600
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunohistochemistry (IHC)

(Anti-NF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunofluorescence (IF)

(Immunofluorescence of HepG2 cells, using NF1 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HepG2 cells, using NF1 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-NF1 antibody

Mutations linked to neurofibromatosis type 1 led to the identification of NF1. NF1 encodes the protein neurofibromin, which appears to be a negative regulator of the ras signal transduction pathway. In addition to type 1 neurofibromatosis, mutations in NF1 can also lead to juvenile myelomonocytic leukemia. Alternatively spliced NF1 mRNA transcripts have been isolated, although their functions, if any, remain unclear.

NCBI and Uniprot Product Information

• NCBI GI #: 4557793
• NCBI GeneID: 4763
• NCBI Accession #: NP_000258.1
• NCBI GenBank Nucleotide #: NM_000267.3
• UniProt Accession #: P21359; O00662; Q14284; Q14930; Q14931; Q9UMK3
• Molecular Weight: 318,992 Da
• NCBI Official Full Name: neurofibromin isoform 2
• NCBI Official Synonym Full Names: neurofibromin 1
• NCBI Official Symbol: NF1
• NCBI Official Synonym Symbols: WSS; NFNS; VRNF
• NCBI Protein Information: neurofibromin; neurofibromatosis-related protein NF-1
• UniProt Protein Name: Neurofibromin
• Protein Family: Neurofibromin
• UniProt Gene Name: NF1
• UniProt Entry Name: NF1_HUMAN

NCBI Description

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; Nucleolus; Motility/polarity/chemotaxis; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: membrane; axon; dendrite; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylcholine binding

Biological Process: negative regulation of Rac protein signal transduction; neural tube development; negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; Schwann cell development; collagen fibril organization; positive regulation of apoptosis; heart development; negative regulation of transcription factor import into nucleus; negative regulation of neuroblast proliferation; regulation of glial cell differentiation; positive regulation of adenylate cyclase activity; negative regulation of neurotransmitter secretion; skeletal muscle development; adrenal gland development; negative regulation of cell-matrix adhesion; forebrain morphogenesis; regulation of synaptic transmission, GABAergic; myelination in the peripheral nervous system; camera-type eye morphogenesis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; negative regulation of endothelial cell proliferation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; cerebral cortex development; actin cytoskeleton organization and biogenesis; metanephros development; forebrain astrocyte development; regulation of long-term neuronal synaptic plasticity; negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; wound healing; regulation of cell-matrix adhesion; regulation of blood vessel endothelial cell migration; smooth muscle development; sympathetic nervous system development; positive regulation of neuron apoptosis; negative regulation of Ras protein signal transduction; visual learning; cell communication; negative regulation of cell migration; regulation of bone resorption; negative regulation of MAPKKK cascade; MAPKKK cascade; liver development; regulation of angiogenesis; peripheral nervous system development; osteoblast differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of angiogenesis; pigmentation; spinal cord development; negative regulation of astrocyte differentiation; Ras protein signal transduction; response to hypoxia; artery morphogenesis; brain development; cognition

Disease: Neurofibromatosis, Familial Spinal; Watson Syndrome; Juvenile Myelomonocytic Leukemia; Neurofibromatosis, Type I; Neurofibromatosis-noonan Syndrome