SDS-Page
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: NIH/3T3 cell lysate, Primary antibody:NF1 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second)
Rabbit NF1 Polyclonal Antibody | anti-NF1 antibody
NF1 Antibody
Immunohistochemistry: 1: 20-100
SDS-Page
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: NIH/3T3 cell lysate, Primary antibody:NF1 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second)
SDS-Page
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: NIH/3T3 cell lysate, Primary antibody:NF1 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second)
Immunohistochemistry (IHC)
(The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using NF1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200))
Immunohistochemistry (IHC)
(The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using NF1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200))
NCBI and Uniprot Product Information
NCBI Description
This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.
Protein type: GAPs; GAPs, Ras; Motility/polarity/chemotaxis; Nucleolus; Tumor suppressor
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: axon; cytoplasm; cytosol; dendrite; membrane; nucleus
Molecular Function: GTPase activator activity; phosphatidylcholine binding; phosphatidylethanolamine binding; protein binding
Biological Process: actin cytoskeleton organization and biogenesis; adrenal gland development; artery morphogenesis; brain development; camera-type eye morphogenesis; cell communication; cerebral cortex development; cognition; collagen fibril organization; extracellular matrix organization and biogenesis; forebrain astrocyte development; forebrain morphogenesis; heart development; liver development; MAPKKK cascade; metanephros development; myelination in the peripheral nervous system; negative regulation of cell migration; negative regulation of endothelial cell proliferation; negative regulation of fibroblast proliferation; negative regulation of MAP kinase activity; negative regulation of MAPKKK cascade; negative regulation of neuroblast proliferation; negative regulation of oligodendrocyte differentiation; negative regulation of protein kinase activity; negative regulation of Ras protein signal transduction; negative regulation of transcription factor import into nucleus; osteoblast differentiation; peripheral nervous system development; phosphoinositide 3-kinase cascade; pigmentation; positive regulation of adenylate cyclase activity; positive regulation of apoptosis; positive regulation of GTPase activity; positive regulation of neuron apoptosis; Ras protein signal transduction; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of bone resorption; regulation of cell-matrix adhesion; regulation of glial cell differentiation; regulation of GTPase activity; response to hypoxia; Schwann cell development; smooth muscle development; spinal cord development; sympathetic nervous system development; visual learning; wound healing
Disease: Juvenile Myelomonocytic Leukemia; Neurofibromatosis, Familial Spinal; Neurofibromatosis, Type I; Neurofibromatosis-noonan Syndrome; Watson Syndrome
Research Articles on NF1
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Product Notes
The NF1 nf1 (Catalog #AAA9417384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 1:200-1000 Immunohistochemistry: 1: 20-100. Researchers should empirically determine the suitability of the NF1 nf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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