Rabbit MAP Kinase p44/42 Monoclonal Antibody | anti-MAPK1 antibody

MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas

Cat. Num. AAA600367

• Reactivity: Drosophila, Human, Monkey, Mouse, Porcine, Rat0
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: MAP Kinase p44/42; Monoclonal Antibody; phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1; Mitogen-activated Protein Kinase 1; MAP Kinase 1; MAPK 1; ERT1; Extracellular Signal-regulated Kinase 2; ERK-2; MAP Kinase Isoform p42; p42-MAPK; Mitogen-activated Protein Kinase 2; MAP Kinas; Anti -MAP Kinase p44/42; anti-MAPK1 antibody

• Host: Rabbit
• Reactivity: Drosophila, Human, Monkey, Mouse, Porcine, Rat0
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9B152
• Specificity: Recognizes endogenous levels of human p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. Does not crossreact with corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase. Species Crossreactivity: mouse, rat, monkey, mink, porcine, Drosophila, zebrafish
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MAPK1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and Flow Cytometry.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.; Immunofluorescence (IF-IC): 1:200; Flow Cytometry: 1:200
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using MBS600367 (upper), M2352-05E (middle), and MBS600367.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of U0126-inhibited (blue) or PMA-stimulated (green) Jurkat cells, using MBS600367 compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent images of NIH/3T3 cells untreated (left), inhibited with U0126 (MEK1/2 Inhibitor) (center), or stimulated with Platelet-Derived Growth Factor (PDGF) (right) and labeled with MBS600367 (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).)

Related Product Information for anti-MAPK1 antibody

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the identification and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

Product Categories/Family for anti-MAPK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 45383812
• NCBI GeneID: 373953
• NCBI Accession #: NP_989481.1
• NCBI GenBank Nucleotide #: NM_204150.1
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 1
• NCBI Official Symbol: MAPK1
• NCBI Protein Information: mitogen-activated protein kinase 1