Mouse anti-Human CENPB Polyclonal Antibody | anti-Cenpb antibody

CENPB (Major Centromere Autoantigen B, Centromere Protein B, CENP-B)

Cat. Num. AAA6002744

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CENPB; Polyclonal Antibody; CENPB (Major Centromere Autoantigen B; Centromere Protein B; CENP-B); Anti -CENPB (Major Centromere Autoantigen B; anti-Cenpb antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CENPB.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS

• Applicable Applications for anti-Cenpb antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human CENPB, aa1-599 (AAH53847.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CENPB polyclonal antibody. Western Blot analysis of CENPB expression in A-431.)

Western Blot (WB)

(Western Blot analysis of CENPB expression in transfected 293T cell line by CENPB polyclonal antibody. Lane1:CENPB transfected lysate (65.89kD). Lane2:Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to CENPB on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-Cenpb antibody

This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Product Categories/Family for anti-Cenpb antibody

Antibodies; Abs to Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 13879308
• NCBI GeneID: 12616
• UniProt Accession #: P27790; Q7TSG8
• Molecular Weight: 65,381 Da
• NCBI Official Full Name: Cenpb protein
• NCBI Official Synonym Full Names: centromere protein B
• NCBI Official Symbol: Cenpb
• NCBI Protein Information: major centromere autoantigen B; CENP-B
• UniProt Protein Name: Major centromere autoantigen B
• Protein Family: CENPB DNA-binding domain-containing protein
• UniProt Gene Name: Cenpb
• UniProt Synonym Gene Names: Cenp-b; CENP-B
• UniProt Entry Name: CENPB_MOUSE

Uniprot Description

CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Protein type: DNA-binding

Cellular Component: chromosome; nucleus; chromosome, pericentric region

Molecular Function: nucleic acid binding; DNA binding; chromatin binding

Biological Process: regulation of transcription, DNA-dependent