Western Blot (WB)
(Western blot detection of Glutaminase in K562,3T3,Hela cell lysates using Glutaminase Rabbit mAb(1:1000 diluted). Predicted band size:74kDa. Observed band size:68kDa.)
Rabbit Glutaminase Monoclonal Antibody | anti-GLS antibody
Glutaminase Rabbit mAb
ICC/IF: 1:50
FC/FACS: 1:50
IP: 1:20
Western Blot (WB)
(Western blot detection of Glutaminase in K562,3T3,Hela cell lysates using Glutaminase Rabbit mAb(1:1000 diluted). Predicted band size:74kDa. Observed band size:68kDa.)
Western Blot (WB)
(Western blot detection of Glutaminase in K562,3T3,Hela cell lysates using Glutaminase Rabbit mAb(1:1000 diluted). Predicted band size:74kDa. Observed band size:68kDa.)
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.CautionIsoform 3 is predicted to be expressed at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay (PubMed:14759258), but has been shown to be well expressed (PubMed:17940881 and PubMed:11015561) and the encoded protein is detected in mitochondria (PubMed:22228304).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; EC 3.5.1.2
Chromosomal Location of Human Ortholog: 2q32-q34
Cellular Component: mitochondrion; mitochondrial matrix; cytosol
Molecular Function: protein binding; glutaminase activity
Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization
Research Articles on GLS
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Product Notes
The GLS gls (Catalog #AAA4752773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glutaminase Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutaminase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 ICC/IF: 1:50 FC/FACS: 1:50 IP: 1:20. Researchers should empirically determine the suitability of the GLS gls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutaminase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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