Rat anti-Mouse CD180 Monoclonal Antibody | anti-CD180 antibody
CD180, Mouse, mAb RP/14
Gene Names
CD180; LY64; Ly78; RP105
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD180; Monoclonal Antibody; Mouse; mAb RP/14; RP105; Ly78; anti-CD180 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
661
Applicable Applications for anti-CD180 antibody
Flow Cytometry (FC/FACS), FS
Application Notes
Application Notes: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-CD180 antibody
Product Description: Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.
References
1. Miyake, K et al; Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 1998, 161: 1348 2. Nagai, Y et al; Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 2002, 99: 1699
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,179 Da
NCBI Official Full Name
CD180 antigen
NCBI Official Synonym Full Names
CD180 molecule
NCBI Official Symbol
CD180
NCBI Official Synonym Symbols
LY64; Ly78; RP105
NCBI Protein Information
CD180 antigen; lymphocyte antigen 64; radioprotective 105 kDa protein; lymphocyte antigen-64, radioprotective, 105kDa
UniProt Protein Name
CD180 antigen
Protein Family
UniProt Gene Name
CD180
UniProt Synonym Gene Names
LY64; RP105
UniProt Entry Name
CD180_HUMAN
NCBI Description
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]
Uniprot Description
CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q12
Cellular Component: integral to membrane; plasma membrane
Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response
Research Articles on CD180
Similar Products
Product Notes
The CD180 cd180 (Catalog #AAA584021) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD180, Mouse, mAb RP/14 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD180 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FS. Application Notes: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the CD180 cd180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD180, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
