Rabbit anti-Human MLL5 Polyclonal Antibody | anti-MLL5 antibody

MLL5, NT (MLL5, KMT2E, Histone-lysine N-methyltransferase MLL5, Lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5) (Azide free) (HRP)

Cat. Num. AAA6321145

• Gene Names: KMT2E; MLL5; NKp44L; ODLURO; HDCMC04P
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MLL5; Polyclonal Antibody; NT (MLL5; KMT2E; Histone-lysine N-methyltransferase MLL5; Lysine N-methyltransferase 2E; Myeloid/lymphoid or mixed-lineage leukemia protein 5) (Azide free) (HRP); Myeloid/lymphoid or mixed-lineage leukemia protein 5; anti-MLL5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1858

• Applicable Applications for anti-MLL5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MLL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human MLL5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MLL5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MLL5 antibody detected the MLL5 protein (arrow).)

Related Product Information for anti-MLL5 antibody

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq].

Product Categories/Family for anti-MLL5 antibody

Antibodies; Enzymes; Methyltransferase

References

Liu, J., et al. J. Biol. Chem. 285(27):20904-20914(2010). Fujiki, R., et al. Nature 459(7245):455-459(2009). Cheng, F., et al. Int. J. Biochem. Cell Biol. 40(11):2472-2481(2008). Sun, X.J., et al. PLoS ONE 3 (1), E1499 (2008): Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 33636768
• NCBI GeneID: 55904
• NCBI Accession #: NP_891847.1; NP_061152.3
• NCBI GenBank Nucleotide #: NP_891847.1
• UniProt Accession #: Q8NFF8; Q8IZD2
• NCBI Official Full Name: inactive histone-lysine N-methyltransferase 2E
• NCBI Official Synonym Full Names: lysine methyltransferase 2E (inactive)
• NCBI Official Symbol: KMT2E
• NCBI Official Synonym Symbols: MLL5; NKp44L; ODLURO; HDCMC04P
• NCBI Protein Information: inactive histone-lysine N-methyltransferase 2E

NCBI Description

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]