Cortisol ELISA Kit
Cortisol ELISA Kit
Synonyms
Cortisol; Cortisol ELISA Kit; Cortisol ELISA; Cortisol elisa kit
Assay Type
Competitive
Sensitivity
1.0 pg/mL
Intra-assay Precision
Within run variation was determined by replicate (16x) the measurement of two different saliva control in one assay. The within assay variability is <= 9.7%
Inter-assay Precision
Between run variation was determined by replicate (10x) the measurement of two different saliva control with different lots of kit. The between assay variability is <= 13.7%.
Preparation and Storage
Shelf Life: 18 Months
Related Product Information for Cortisol elisa kit
Intended Uses: Competitive immunoenzymatic colorimetric method for the determination of Estriol in saliva. For research use only.
Principle of the Assay: The Estriol (antigen) in the sample competes with the antigenic Estriol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Estriol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution is added. The colour intensity is inversely proportional to the Estriol concentration in the sample. Estriol concentration in the sample is calculated through a calibration curve!!Background/Introduction: Estriol (also Oestriol) is one of the three main estrogens produced by the human body. It is only produced in significant amounts during pregnancy as it is made by the fetus. During pregnancy the production of estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non-pregnant levels. In the maternal circulation, estriol undergoes a rapid conjugation in the liver followed by urinary excretion with a half-life of about 20 minutes. Since normal estriol production depends on an intact maternal-placental-fetal circulation and functional fetal metabolism, maternal estriol levels have been used to monitor fetal status during pregnancy, particularly during the third trimester. DHEA-S is produced by the adrenal cortex of the fetus, this is converted to estriol by the placenta. If levels are abnormally low in a pregnant woman, this may indicate a problem with the development in the child. Levels of estriol in non-pregnant women do not change much after menopause, and levels are not significantly different from levels in men.
Principle of the Assay: The Estriol (antigen) in the sample competes with the antigenic Estriol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Estriol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution is added. The colour intensity is inversely proportional to the Estriol concentration in the sample. Estriol concentration in the sample is calculated through a calibration curve!!Background/Introduction: Estriol (also Oestriol) is one of the three main estrogens produced by the human body. It is only produced in significant amounts during pregnancy as it is made by the fetus. During pregnancy the production of estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non-pregnant levels. In the maternal circulation, estriol undergoes a rapid conjugation in the liver followed by urinary excretion with a half-life of about 20 minutes. Since normal estriol production depends on an intact maternal-placental-fetal circulation and functional fetal metabolism, maternal estriol levels have been used to monitor fetal status during pregnancy, particularly during the third trimester. DHEA-S is produced by the adrenal cortex of the fetus, this is converted to estriol by the placenta. If levels are abnormally low in a pregnant woman, this may indicate a problem with the development in the child. Levels of estriol in non-pregnant women do not change much after menopause, and levels are not significantly different from levels in men.
Product Categories/Family for Cortisol elisa kit
Similar Products
Product Notes
The Cortisol (Catalog #AAA580005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cortisol, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
