Typical Testing Data/Standard Curve (for reference only)
Rat CRP ELISA Kit | CRP elisa kit
CRP (Rat) ELISA
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Rat C-Reactive Protein Assay is species specific and provides a quantitative assessment of CRP levels in rat serum or plasma. The Rat C-Reactive Protein Assay is a sandwich-based enzyme linked immunoassay in which the microtiter plates are coated with pneumococcal C-polysaccharide. After samples are prepared following the listed specimen collection procedure, they are applied to the antigen coated plate alongside the prepared standards. After incubation, the wells are decanted and washed to remove unreacted serum or plasma proteins, and an enzyme labeled anti-rat CRP antibody (conjugate) is added and reacts with the antigen-antigen complexes. Following another incubation period, the wells are decanted and washed to remove unreacted conjugate. A hydrogen peroxide substrate with TMB as a chromogen is added to start color development. The intensity of the color is directly proportional to the amount of rat CRP in the sample. Therefore, the greater the intensity of the blue color, the higher the rat CRP concentration in the sample. The reaction is interrupted with a stop solution that turns the blue product yellow. The absorbance is read at a wavelength of 450nm on a spectrophotometer or plate reader.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]
Uniprot Description
CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q23.2
Cellular Component: extracellular space; growth cone; extracellular region; filopodium
Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; low-density lipoprotein binding; virion binding; cholesterol binding; calcium ion binding
Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; response to lead ion; acute-phase response; response to hypoxia; opsonization; inflammatory response; complement activation, classical pathway; aging
Research Articles on CRP
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Product Notes
The Rat CRP crp (Catalog #AAA494518) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494518 ELISA Kit recognizes Rat CRP. It is sometimes possible for the material contained within the vial of "CRP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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