Mouse anti-Human, Rat CytokeRatin 17 / KeRatin K17 Monoclonal Antibody | anti-KRT17 antibody

Mouse anti CytokeRatin 17 / KeRatin K17

Cat. Num. AAA570068

• Gene Names: KRT17; PC; K17; PC2; PCHC1
• Reactivity: Human, Rat
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: CytokeRatin 17 / KeRatin K17; Monoclonal Antibody; Mouse anti CytokeRatin 17 / KeRatin K17; anti-KRT17 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: E3
• Specificity: E3 reacts with cytokeRatin 17 in basal layers of pseudo-stRatified and transitional epithelia.
• Form/Format: Each vial contains 100 ug 1 mg/ml purified monoclonal antibody in PBS 0.02% sodium azide.

• Applicable Applications for anti-KRT17 antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
• Application Notes: E3 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
• Source Note: E3 is a Mouse monoclonal IgG2b antibody derived by fusion of X63 Ag 8.653 Mouse myeloma cells with spleen cells from a Balb/c Mouse immunized with a cytoskeletal prepaRation from Rat colon.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-KRT17 antibody

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Product Categories/Family for anti-KRT17 antibody

Cytoskeletal; Cell differentiation; Cytoskeleton

References

Guelstein, V. I., Tchypysheva, T. A., Ermilova, V. D., Litvinova, L. V., Troyanovsky, S. M., Bannikov, G. A. (1988). Monoclonal antibody mapping of keRatins 8 and 17 and of vimentin in normal Human mammary gland, benign tumors, dysplasias and breast cancer, Int J Cancer 42, 147-53. Troyanovsky, S. M., Guelstein, V. I., Tchipysheva, T. A., Krutovskikh, V. A., Bannikov, G. A. (1989). Patterns of expression of keRatin 17 in Human epithelia: dependency on cell position, J Cell Sci 93, 419-26. Wetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related keRatins in Human breast cancer. Am J Pathol 138, 751-63. Moll, I., Moll, R. (1991). CompaRative cytokeRatin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300-09. De Jong, E., van Vlijmen, I., van Erp, P., Ramaekers, F., Troyanowsky, S., Van de Kerkhof, P. (1991). Monoclonal anti-keRatin 17: A useful marker for anti-psoriatic therapies. Arch Dermatol Res 283, 480-82. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., and Vooijs, P. (1992). KeRatin expression in cervical cancer, Am J Pathol 141, 497-511. Wetzels, R. H., Schaafsma, H. E., Leigh, I. M., Lane, E. B., Troyanovsky, S. M., Wagenaar, S. S., Vooijs, G. P., and Ramaekers, F. C. (1992). Laminin and type VII collagen distribution in different types of Human lung carcinoma: correlation with expression of keRatins 14, 16, 17 and 18. Histopathology 20, 295-303. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Robben, H., Lane, B., Leigh, I., Plantema, F., and Vooijs, P. (1992). Basal-cell keRatins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia. Am J Pathol 140, 601-12. Smedts, F., Ramaekers, F., Link, M., Lauerova, L., Troyanovsky, S., Schijf, C., and Vooijs, G. P. (1994). Detection of keRatin subtypes in routinely processed cervical tissue: impliCations for tumour classifiCation and the study of cervix cancer aetiology. Virchows Arch 425, 145-155. Demirkesen, C., Hoede, N., Moll, R. (1995). Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeRatins. J Cutan Pathol 22, 518-35. Moll, R., et al. (1995). Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W Zuckschwerdt Verlag. Litvinov, S. V., van Driel, W., van Rhijn, C. M., Bakker, H. A., van Krieken, H., Fleuren, G. J., and Warnaar, S. O. (1996). Expression of Ep-CAM in cervical squamous epithelia correlates with an increased prolifeRation and the disappearance of markers for terminal differentiation. Am J Pathol 148, 865-75.

NCBI and Uniprot Product Information

• NCBI GI #: 30379
• NCBI GeneID: 3872
• UniProt Accession #: Q04695; Q6IP98; Q8N1P6; A5Z1M9; A5Z1N0; A5Z1N1; A5Z1N2; A6NDV6; A6NKQ2; Q14666
• Molecular Weight: 48,106 Da
• NCBI Official Full Name: cytokeratin 17
• NCBI Official Synonym Full Names: keratin 17
• NCBI Official Symbol: KRT17
• NCBI Official Synonym Symbols: PC; K17; PC2; PCHC1
• NCBI Protein Information: keratin, type I cytoskeletal 17; 39.1; CK-17; keratin-17; cytokeratin-17; OTTHUMP00000164770
• UniProt Protein Name: Keratin, type I cytoskeletal 17
• Protein Family: Keratin
• UniProt Gene Name: KRT17
• UniProt Entry Name: K1C17_HUMAN

NCBI Description

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq]

Uniprot Description

K17: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; MHC class II receptor activity; structural constituent of cytoskeleton; MHC class II protein binding

Biological Process: positive regulation of hair follicle development; epidermis development; hair follicle morphogenesis; positive regulation of translation; keratinization; intermediate filament organization; signal transduction; positive regulation of cell growth

Disease: Steatocystoma Multiplex; Pachyonychia Congenita 2