Typical Testing Data/Standard Curve (for reference only)
Mouse NADPH oxidase 1 ELISA Kit | Nox1 elisa kit
Mouse NADPH oxidase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of the NADPH oxidase enzyme, which is a membrane-bound, multisubunit protein. This complex catalyzes the transfer of electrons from NADPH to molecular oxygen to produce superoxide or hydrogen peroxide and may have a role in antimicrobial killing and cell signaling. This protein binds other subunits in order to produce superoxide. In mouse, deficiency of this enzyme may be associated with changes in blood pressure. [provided by RefSeq, Jun 2013]
Uniprot Description
NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Oxidoreductase; Channel, misc.; EC 1.-.-.-
Cellular Component: cell junction; cell projection; cytoplasm; early endosome; endosome; integral to membrane; membrane; NADPH oxidase complex; plasma membrane
Molecular Function: F420H2 dehydrogenase activity; malolactic enzyme activity; metal ion binding; N-ethylmaleimide reductase activity; oxidoreductase activity; Rac GTPase binding; sulfur oxygenase reductase activity; superoxide-generating NADPH oxidase activity; voltage-gated proton channel activity
Biological Process: angiogenesis; cell migration; extracellular matrix organization and biogenesis; hydrogen peroxide metabolic process; intracellular pH elevation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of smooth muscle cell proliferation; proton transport; regulation of systemic arterial blood pressure by renin-angiotensin; response to reactive oxygen species; signal transduction; superoxide metabolic process; superoxide release; transport
Research Articles on Nox1
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Product Notes
The Mouse Nox1 nox1 (Catalog #AAA2885477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885477 ELISA Kit recognizes Mouse Nox1. It is sometimes possible for the material contained within the vial of "NADPH oxidase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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