Wnt-3a active protein
Wnt-3a (mouse), (recombinant)
Gene Names
Wnt3a; vt; Wnt-3a
Purity
>75% by SDS-PAGE; >75% by HPLC analyses
Synonyms
Wnt-3a; Wnt-3a (mouse); (recombinant); Wnt-3a active protein
Host
Cell culture
Purity/Purification
>75% by SDS-PAGE; >75% by HPLC analyses
Form/Format
Lyophilized solid
Endotoxin
Less than 0.2 ng perug/ (2EU/ug)
Biological Activity
Wnt-3a can induce beta-catenin mediated response in a TCF/LEF reporter gene luciferase assay
Reconstitution
Prepare 5ug/mL stock solution of Wnt3a by adding 200uL deionized water into the vial of lyophilized Wnt3a
Preparation and Storage
For long term storage, store at -80 degree C
Stable for at least 12 months after receipt when stored, as supplied, at -80 degree C. Following reconstitution, stable for three months when stored at or below -20 degree C
Shipping: Shipped on Dry Ice
Stable for at least 12 months after receipt when stored, as supplied, at -80 degree C. Following reconstitution, stable for three months when stored at or below -20 degree C
Shipping: Shipped on Dry Ice
Related Product Information for Wnt-3a active protein
Wnt expression and signal transduction have been identified as critical components in regulating embryogenesis, cell proliferation, and bone, glucose and lipid metabolism. Wnt-3a signalling also plays a role in regulating the differentation of stem cells. Wnt-3a is a member of the highly conserved, structurally related cysteine-rich Wnt family glycoproteins important for normal developmental processes and oncogenesis. Wnt-3a proteins are secreted and bind to receptors of the Frizzled and LRP families on the cell surface, which leads to the stabilization of beta-catenin and increase of beta-catenin translocation into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. The predicted mouse Wnt-3a protein of 352 amino acid residues and contains an N-terminal signal peptide for secretion. It shares 96% amino acid identity to human Wnt-3a protein. Due to glycosylation, it migrates at an apparent molecular weight of ~40 kDa by SDS-PAGE analysis under reducing conditions. .
Product Categories/Family for Wnt-3a active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,258 Da
NCBI Official Full Name
protein Wnt-3a
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol
Wnt3a
NCBI Official Synonym Symbols
vt; Wnt-3a
NCBI Protein Information
protein Wnt-3a
UniProt Protein Name
Protein Wnt-3a
Protein Family
UniProt Gene Name
Wnt3a
UniProt Synonym Gene Names
Wnt-3a
Uniprot Description
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Research Articles on Wnt-3a
Similar Products
Product Notes
The Wnt-3a wnt3a (Catalog #AAA566755) is an Active Protein produced from Cell culture and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Wnt-3a, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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