Human Bleomycin Hydrolase (BLMH) ELISA Kit | BLMH elisa kit

Human Bleomycin Hydrolase (BLMH) ELISA Kit

Cat. Num. AAA450693

• Gene Names: BLMH; BH; BMH
• Reactivity: Human
• Synonyms: Bleomycin Hydrolase (BLMH); Human Bleomycin Hydrolase (BLMH) ELISA Kit; BH; BMH; BLMH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BLMH. No significant cross-reactivity or interference between BLMH and analogues was observed.
• Sequence Length: 455

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.071ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BLMH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BLMH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BLMH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BLMH in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BLMH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BLMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BLMH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BLMH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557367
• NCBI GeneID: 642
• NCBI Accession #: NP_000377.1
• NCBI GenBank Nucleotide #: NM_000386.3
• UniProt Accession #: Q13867; Q53F86; Q9UER9; B2R796
• Molecular Weight: 52,562 Da
• NCBI Official Full Name: bleomycin hydrolase
• NCBI Official Synonym Full Names: bleomycin hydrolase
• NCBI Official Symbol: BLMH
• NCBI Official Synonym Symbols: BH; BMH
• NCBI Protein Information: bleomycin hydrolase
• UniProt Protein Name: Bleomycin hydrolase
• Protein Family: Bleomycin hydrolase
• UniProt Gene Name: BLMH
• UniProt Synonym Gene Names: BH; BLM hydrolase; BMH

NCBI Description

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.