GRO-gamma alpha (CXCL3) Recombinant Protein | CXCL3 recombinant protein
Rat GRO-gamma alpha (CXCL3) Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
GRO-gamma alpha (CXCL3); Rat GRO-gamma alpha (CXCL3) Recombinant; Rat GRO-g recombinant; GRO-gamma; GROg; cxcl3; mip2; mip-2; CXCL3 recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant mouse GRO-gamma/CXCL3 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 40mM NaCl, 10mM PB, pH 7.0.
Sequence
MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL
Sequence Length
100
Domain
IL8
Host Note
Optimized DNA sequence encoding rat GRO-gamma/CXCL3 mature chain was expressed in Escherichia Coli
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Molecular Weight Note
Native rat GROgamma/CXCL3 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.
Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for CXCL3 recombinant protein
Growth regulated oncogene- gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.
Product Categories/Family for CXCL3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,109 Da
NCBI Official Full Name
C-X-C motif chemokine 3
UniProt Protein Name
C-X-C motif chemokine 3
Protein Family
UniProt Gene Name
Cxcl3
UniProt Synonym Gene Names
Cinc2; CINC-2
UniProt Entry Name
CXCL3_RAT
NCBI Description
acts as a chemoattractant for neutrophils; involved in the inflammatory response [RGD, Feb 2006]
Uniprot Description
CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; CXCR chemokine receptor binding
Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; hemopoiesis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; inflammatory response; chemotaxis; regulation of cell proliferation
Similar Products
Product Notes
The CXCL3 cxcl3 (Catalog #AAA553260) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL. It is sometimes possible for the material contained within the vial of "GRO-gamma alpha (CXCL3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
