PDGFA recombinant protein

PDGFA

Cat. Num. AAA517479

• Gene Names: PDGFA; PDGF1; PDGF-A
• Applications: Cell Assay
• Synonyms: PDGFA; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; PDGF-AA; PDGF1; Recombinant Human Platelet-Derived Growth Factor Subunit A (PDGFA); PDGFA recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in Tris and NaCl

• Applicable Applications for PDGFA recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The ED50 as determined by the dose-dependent proliferation of mouse embryonic fibroblast cells was found to be )

SDS-Page

(Purity: Recombinant Human PDGFA resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for PDGFA recombinant protein

Recombinant Human PDGFA was expressed in E Coli cells. The protein accession number is P04085
The PDGF family is comprised of five different disulphide-linked dimers of four different polypeptide chains: A, B, C and D (PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD). Synthesized mainly by megakaryocytes, PDGFs are stored in the alpha granules of platelets from which they are released following platelet activation. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells and endothelial cells. Functioning as an autocrine and paracrine growth factor, PDGFs are involved in a number of biological processes that include but not limited to hyperplasia, chemotaxis, embryonic neuron development, wound healing and respiratory tubule epithelial cell development. Aberrant expression of PDGFs is observed with vascular proliferative diseases such as atherosclerosis. PDGFs regulate the synthesis of their own receptor and also influence the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL and transferrin. Recombinant Human PDGFA is a disulfide-linked homodimer of two A chains.

Product Categories/Family for PDGFA recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 77695917
• NCBI GeneID: 5154
• NCBI Accession #: NP_002598.4
• NCBI GenBank Nucleotide #: NM_002607.5
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 24,043 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet-derived growth factor alpha polypeptide
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; microvillus; cell surface; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; hair follicle development; positive regulation of MAPKKK cascade; cell-cell signaling; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; inner ear development; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; response to inorganic substance; phosphoinositide-mediated signaling; response to retinoic acid; fibroblast growth factor receptor signaling pathway; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration