HtrA2/Omi recombinant protein

HtrA2/Omi, 134-458aa, Human, His tag, E Coli

Cat. Num. AAA203109

• Gene Names: HTRA2; OMI; MGCA8; PARK13; PRSS25
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HtrA2/Omi; 134-458aa; Human; His tag; E Coli; High temperature requirement protein A2; HTRA2; OMI; PRSS25; EC 3.4.21.108; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; Serine protease HTRA2; mitochondrial; HtrA2/Omi recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0), 50 mM NaCl, 1 mM DTT, 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH
• Sequence Length: 458

• Applicable Applications for HtrA2/Omi recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HtrA2/Omi recombinant protein

HtrA2/Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death. Mature form of HtrA2/Omi (residues 134-458) was overexpressed in E Coli, and purified by conventional column chromatography techniques.

Product Categories/Family for HtrA2/Omi recombinant protein

Enzymes & Proteases

References

Savopoulos JW., et al., (2000) Protein Expr. Purif. 19, 227-34; Gray CW, et al., (2000) Eur. J. Biochem. 267, 5699-710 ; Martins LM, et al., (2002) J. Biol. Chem. 277, 439-44; Van Loo G, et al., (2002) Cell Death Differ. 9, 20-60;

NCBI and Uniprot Product Information

• NCBI GI #: 7019477
• NCBI GeneID: 27429
• NCBI Accession #: NP_037379
• NCBI GenBank Nucleotide #: NM_013247.4
• UniProt Accession #: O43464; Q9HBZ4; Q9P0Y3; Q9P0Y4
• Molecular Weight: 36kDa (334aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: serine protease HTRA2, mitochondrial isoform 1 preproprotein
• NCBI Official Synonym Full Names: HtrA serine peptidase 2
• NCBI Official Symbol: HTRA2
• NCBI Official Synonym Symbols: OMI; MGCA8; PARK13; PRSS25
• NCBI Protein Information: serine protease HTRA2, mitochondrial
• UniProt Protein Name: Serine protease HTRA2, mitochondrial
• UniProt Gene Name: HTRA2
• UniProt Synonym Gene Names: OMI; PRSS25; HtrA2

NCBI Description

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.