GM-CSF (CSF2) Recombinant Protein | GM-CSF recombinant protein

GM-CSF (CSF2)

Cat. Num. AAA517030

• Gene Names: CSF2; GMCSF
• Applications: Cell Assay
• Synonyms: GM-CSF (CSF2); GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF-2; Pluripoietin-alpha; Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2); GM-CSF recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in Tris and NaCl

• Applicable Applications for GM-CSF recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Sample Cell Assay Data: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be )

SDS-Page

(Purity: Recombinant Human GM-CSF (CSF2) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for GM-CSF recombinant protein

Recombinant Human GM-CSF (CSF2) was expressed in E Coli cells. The protein accession number is P04141
GM-CSF is a hematopoietic growth factor that is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes following their activation by antigens or mitogens. GM-CSF stimulates the development of macrophages and neutrophils and plays a role in the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo compared to its non-glycosylated counterpart. Human and murine GM-CSF are species-specific and therefore, exhibit no cross-reactivity. Recombinant Human GM-CSF is a non-glycosylated, monomeric protein that contains intra-chain disulfide bonds.

Product Categories/Family for GM-CSF recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN