SDS-Page
(Purity: This is the sample data. For lot specific data please refer to the respective datasheet.)
COVID 19 NSP8 Coronavirus Recombinant Protein | COVID-19 recombinant protein
2019-nCoV NSP8 Protein
Synonyms
COVID 19 NSP8 Coronavirus; 2019-nCoV NSP8 Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP8; NSP8: Non-structural protein 8; COVID-19 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 20mM Tris, pH7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol.
Tag
HIS
Species
SARS-CoV-2
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: This is the sample data. For lot specific data please refer to the respective datasheet.)
SDS-Page
(Purity: This is the sample data. For lot specific data please refer to the respective datasheet.)
Related Product Information for COVID-19 recombinant protein
Recombinant full-length viral NSP8 was expressed in E Coli using a C-terminal His tag. The gene accession number is QHD43415.
Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). Coronavirus RNA genome encodes a complex replication machinery consisting of 16 viral non-structural proteins (NSPs). NSP7 and NSP8 stabilize regions of NSP12 (RNA-dependent RNA polymerase; RdRp) involved in RNA binding and are essential for a highly active NSP12 polymerase complex. NSP7 has been suggested to confer RNA-binding properties to the trimeric polymerase complex (2). NSP8 has been shown to act as an oligo(U)-templated polyadenylyltransferase and a robust (mono/oligo) adenylate transferase (3). The NSP7/NSP8/NSP12 polymerase complex associates with NSP14 which is required to safeguard coronavirus replication fidelity (2).
Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). Coronavirus RNA genome encodes a complex replication machinery consisting of 16 viral non-structural proteins (NSPs). NSP7 and NSP8 stabilize regions of NSP12 (RNA-dependent RNA polymerase; RdRp) involved in RNA binding and are essential for a highly active NSP12 polymerase complex. NSP7 has been suggested to confer RNA-binding properties to the trimeric polymerase complex (2). NSP8 has been shown to act as an oligo(U)-templated polyadenylyltransferase and a robust (mono/oligo) adenylate transferase (3). The NSP7/NSP8/NSP12 polymerase complex associates with NSP14 which is required to safeguard coronavirus replication fidelity (2).
Product Categories/Family for COVID-19 recombinant protein
References
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. PNAS. U.S.A. 2014, 111: E3900-E3909.
3. Tvarogova J, et al: Identification and characterization of a human coronavirus 229E nonstructural protein 8-associated RNA 3'-terminal adenylyltransferase activity. Virol. 2019, 368: 779-782.
2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. PNAS. U.S.A. 2014, 111: E3900-E3909.
3. Tvarogova J, et al: Identification and characterization of a human coronavirus 229E nonstructural protein 8-associated RNA 3'-terminal adenylyltransferase activity. Virol. 2019, 368: 779-782.
NCBI and Uniprot Product Information
Molecular Weight
approx. MW: 22kDa
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Product Notes
The COVID-19 (Catalog #AAA516672) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 NSP8 Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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