Western Blot (WB)
(Sample Data: Western Blot analysis of extracts from 293 cells using Anti-Src (Lane 1 and 2) and Anti-phospho-Src (Tyr529) (Lane 3 and 4).)
Rabbit Src Polyclonal Antibody | anti-phospho-Src antibody
Anti-phospho-Src (Tyr529)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
Src; Polyclonal Antibody; Anti-phospho-Src (Tyr529); anti-phospho-Src antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the Src protein phosphorylated at tyrosine 529
Purity/Purification
Affinity chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-Src antibody
Western Blot (WB), Immunohistochemistry (IHC)
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western Blot analysis of extracts from 293 cells using Anti-Src (Lane 1 and 2) and Anti-phospho-Src (Tyr529) (Lane 3 and 4).)
Western Blot (WB)
(Sample Data: Western Blot analysis of extracts from 293 cells using Anti-Src (Lane 1 and 2) and Anti-phospho-Src (Tyr529) (Lane 3 and 4).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-Src (Tyr529).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-Src (Tyr529).)
Related Product Information for anti-phospho-Src antibody
SRC family belongs to non-receptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates (1). SRC is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases (2). In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression.
Product Categories/Family for anti-phospho-Src antibody
References
1. Collett, M S. et al: Protein kinase activity associated with the avian sarcoma virus src gene product. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4.
2. Jacobs, C. et al: Expression of pp60c-src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696-702.
3. Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997).Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280.
2. Jacobs, C. et al: Expression of pp60c-src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696-702.
3. Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997).Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280.
Similar Products
Product Notes
The phospho-Src (Catalog #AAA516217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-Src (Tyr529) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Src can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the phospho-Src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
