Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) antibody)
Rabbit Src Polyclonal Antibody | anti-Src antibody
Src antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Src; Polyclonal Antibody; Src antibody; Rabbit polyclonal Src antibody; Polyclonal Src antibody; Anti-Src antibody; c-SRC antibody; p60-Src antibody; Phospho Tyr529 antibody; SRC1 antibody; ASV antibody; anti-Src antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Src antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
Src antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Src (Phospho-Tyr529) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-Src antibody
Src is highly similar to the v-Src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The proteinencoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer.
Product Categories/Family for anti-Src antibody
Similar Products
Product Notes
The Src (Catalog #AAA5309548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Src can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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