Immunohistochemistry (IHC)
(Sample Data: Anti-NOTCH 2 was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.)
Rabbit anti-Human NOTCH 2 Polyclonal Antibody | anti-NOTCH2 antibody
Anti-NOTCH 2
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
NOTCH 2; Polyclonal Antibody; Anti-NOTCH 2; anti-NOTCH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the NOTCH 2 protein
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 0.01% (w/v) Sodium Azide
Applicable Applications for anti-NOTCH2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human NOTCH 2 located near the N-terminal sequence of the cleaved intracellular domain (NICD).
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Immunohistochemistry (IHC)
(Sample Data: Anti-NOTCH 2 was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.)
Immunohistochemistry (IHC)
(Sample Data: Anti-NOTCH 2 was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.)
Related Product Information for anti-NOTCH2 antibody
The Anti-NOTCH 2 antibody recognizes NOTCH 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called NOTCH extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Product Categories/Family for anti-NOTCH2 antibody
References
1. Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW, Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. 2000.
2. Gridley T, Notch signaling and inherited disease syndromes. Hum. Mol. Genet.: 12, Suppl 1, R9-R13. 2003.
2. Gridley T, Notch signaling and inherited disease syndromes. Hum. Mol. Genet.: 12, Suppl 1, R9-R13. 2003.
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Product Notes
The NOTCH2 (Catalog #AAA516116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOTCH 2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOTCH 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the NOTCH2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOTCH 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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