Rabbit anti-Human NOTCH 2 Polyclonal Antibody | anti-NOTCH2 antibody

Anti-NOTCH 2 Antibody

Cat. Num. AAA4158616

• Gene Names: NOTCH2; hN2; AGS2; HJCYS
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified
• Synonyms: NOTCH 2; Polyclonal Antibody; Anti-NOTCH 2 Antibody; anti-NOTCH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is nonconservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
• Purity/Purification: Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 2471

• Applicable Applications for anti-NOTCH2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. For western blot experiments, one can expect a band of ≈110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate.
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-NOTCH2 antibody

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

NCBI and Uniprot Product Information

• NCBI GI #: 24041035
• NCBI GeneID: 4853
• NCBI GenBank Nucleotide #: NP_077719.2
• UniProt Accession #: Q04721
• NCBI Official Full Name: neurogenic locus notch homolog protein 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: notch receptor 2
• NCBI Official Symbol: NOTCH2
• NCBI Official Synonym Symbols: hN2; AGS2; HJCYS
• NCBI Protein Information: neurogenic locus notch homolog protein 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH2
• UniProt Synonym Gene Names: Notch 2; hN2
• UniProt Entry Name: NOTC2_HUMAN

NCBI Description

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13-p11

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; nucleus; receptor complex; cilium

Molecular Function: protein binding; receptor activity; calcium ion binding

Biological Process: positive regulation of apoptosis; apoptosis; multicellular organismal development; myeloid dendritic cell differentiation; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; hemopoiesis; cell cycle arrest; cell growth; positive regulation of BMP signaling pathway; embryonic limb morphogenesis; nervous system development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; intracellular receptor-mediated signaling pathway; in utero embryonic development; stem cell maintenance; Notch receptor processing; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; gene expression; positive regulation of Ras protein signal transduction; determination of left/right symmetry; negative regulation of apoptosis

Disease: Hajdu-cheney Syndrome; Alagille Syndrome 2