Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.)
Mouse anti-Mouse, Rat Notch1 Monoclonal Antibody | anti-Notch1 antibody
Notch1 Antibody: Alkaline Phosphatase
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.)
Scientific Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
NCBI and Uniprot Product Information
Uniprot Description
Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.
Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Transcription factor; Oncoprotein
Cellular Component: Golgi apparatus; cell surface; membrane; cell; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane; acrosome; nucleus; receptor complex
Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; calcium ion binding; receptor activity; chromatin binding; transcription factor activity
Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; heart development; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; regulation of cell migration; negative regulation of BMP signaling pathway; neuron differentiation; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of astrocyte differentiation; embryonic limb morphogenesis; positive regulation of keratinocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; cell fate commitment; transcription, DNA-dependent; neuron fate commitment; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; negative regulation of neuron differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; anagen; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; foregut morphogenesis; positive regulation of epithelial cell proliferation; endoderm development; osteoblast fate commitment; transcription from RNA polymerase II promoter; epidermis development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of cell proliferation; glial cell differentiation; regulation of neurogenesis; astrocyte differentiation; regulation of transcription, DNA-dependent; cardiac muscle morphogensis; forebrain development; positive regulation of cell proliferation; angiogenesis; heart looping; regulation of somitogenesis; cell differentiation; positive regulation of BMP signaling pathway; regulation of developmental process; mesenchymal cell development; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; regulation of cell proliferation; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; negative regulation of cell differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; sprouting angiogenesis; negative regulation of myoblast differentiation; auditory receptor cell fate commitment; positive regulation of cell migration; lung development
Research Articles on Notch1
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Product Notes
The Notch1 notch1 (Catalog #AAA801481) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Notch1 Antibody: Alkaline Phosphatase reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Notch1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1:1000 (WB). Researchers should empirically determine the suitability of the Notch1 notch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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