Testing Data
Rabbit anti-Human NOTCH 1 Polyclonal Antibody | anti-NOTCH1 antibody
Anti-Human NOTCH 1 Cleaved N Terminal Antibody
Gene Names
Notch1; TAN1; NOTCH
Reactivity
Human
Applications
ELISA
Purity
Purified
Synonyms
NOTCH 1; Polyclonal Antibody; Anti-Human NOTCH 1 Cleaved N Terminal Antibody; anti-NOTCH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
2531
Applicable Applications for anti-NOTCH1 antibody
ELISA (EIA)
Application Notes
This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-NOTCH1 antibody
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neurogenic locus notch homolog protein 1
NCBI Official Synonym Full Names
notch receptor 1
NCBI Official Symbol
Notch1
NCBI Official Synonym Symbols
TAN1; NOTCH
NCBI Protein Information
neurogenic locus notch homolog protein 1
UniProt Protein Name
Neurogenic locus notch homolog protein 1
Protein Family
UniProt Gene Name
Notch1
UniProt Synonym Gene Names
Notch 1; NEXT; NICD
UniProt Entry Name
NOTC1_RAT
NCBI Description
transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD, Feb 2006]
Research Articles on NOTCH1
Similar Products
Product Notes
The NOTCH1 notch1 (Catalog #AAA4158613) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human NOTCH 1 Cleaved N Terminal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOTCH 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide. Researchers should empirically determine the suitability of the NOTCH1 notch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOTCH 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
