Western Blot (WB)
(Sample Data: Western blot analysis of extracts from Jurkat cells using CHK2 antibody (lanes 1 and 2) and anti-phospho-CHK2 (Thr68) antibody (lanes 3 and 4).)
Rabbit anti-Human CHK2 Polyclonal Antibody | anti-phospho-CHK2 antibody
Anti-phospho-CHK2 (Thr68)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
CHK2; Polyclonal Antibody; Anti-phospho-CHK2 (Thr68); anti-phospho-CHK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the CHK2 protein phosphorylated at threonine 68
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-CHK2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr68
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from Jurkat cells using CHK2 antibody (lanes 1 and 2) and anti-phospho-CHK2 (Thr68) antibody (lanes 3 and 4).)
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from Jurkat cells using CHK2 antibody (lanes 1 and 2) and anti-phospho-CHK2 (Thr68) antibody (lanes 3 and 4).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-phospho-CHK2 (Thr68) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-phospho-CHK2 (Thr68) antibody.)
Related Product Information for anti-phospho-CHK2 antibody
CHK2 is a highly conserved tumor suppressor protein that is activated in response to DNA damage in an ATM-dependent manner (1). Activation of CHK2 is dependent on phosphorylation at sites including Thr68 (2). CHK2 controls cell cycle arrest, DNA repair and apoptosis through activation of DNA damage response proteins such as p53 and BRCA1. Expressing wild-type CHK2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative CHK2 mutant abrogates phosphorylation of p53, leading to decreased stability (3).
Product Categories/Family for anti-phospho-CHK2 antibody
References
1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7.
2. Li J. et al: Regulation of CHK2 by DNA-dependent protein kinase. J Biol Chem. 2005 Mar 25;280(12):12041-50.
3. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.
2. Li J. et al: Regulation of CHK2 by DNA-dependent protein kinase. J Biol Chem. 2005 Mar 25;280(12):12041-50.
3. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.
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Product Notes
The phospho-CHK2 (Catalog #AAA515910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-CHK2 (Thr68) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the phospho-CHK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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