Rabbit anti-Human Chk2 Polyclonal Antibody | anti-CHEK2 antibody

Chk2, phosphorylated (Ser19) (Checkpoint Kinase 2, Rad 53)

Cat. Num. AAA622821

• Gene Names: CHEK2; CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Protein A and peptide affinity chromatography.
• Synonyms: Chk2; Polyclonal Antibody; phosphorylated (Ser19) (Checkpoint Kinase 2; Rad 53); Anti -Chk2; anti-CHEK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of Chk2 only when phosphorylated at Ser19. The antibody does not crossreact with Chk2 phosphorylated at other sites.
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-CHEK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blotting, Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:50
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser19 of human Chk2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of COS cell extracts, control (lane 1), transfected with Wild-type Chk2 (lane 2), Chk2 (S19A) (lane 3), Chk2 (T26S28A) (lane 4), Chk2 (S33S35A) (lane 5) and Chk2 (T68A) (lane 6), using Phospho-Chk2 (Ser19).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of phosphorylated Chk2 in paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-Chk2 (Ser19).)

Western Blot (WB)

(Western blot analysis of extracts from UV-treated Hela cells, using Phospho-Chk2 (Ser19).)

Related Product Information for anti-CHEK2 antibody

Chk2 is the mammalian homologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4). Indeed, after DNA damage by ionizing radiation (IR), UV irradiation and DNA replication blocked by hydroxyurea, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

Product Categories/Family for anti-CHEK2 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 11034732
• NCBI GeneID: 11200
• UniProt Accession #: O96017; Q6QA03; Q6QA04; Q6QA05; Q6QA06; Q6QA07; Q6QA08; Q6QA10; A8K3Y9; B7ZBF3; B7ZBF4; B7ZBF5; Q683Z8; Q9HBS5
• Molecular Weight: 60,915 Da
• NCBI Official Full Name: CHK2
• NCBI Official Synonym Full Names: CHK2 checkpoint homolog (S. pombe)
• NCBI Official Symbol: CHEK2
• NCBI Official Synonym Symbols: CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425
• NCBI Protein Information: serine/threonine-protein kinase Chk2; OTTHUMP00000198969; OTTHUMP00000198970; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; protein kinase CHK2; checkpoint-like protein CHK2
• UniProt Protein Name: Serine/threonine-protein kinase Chk2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: CHEK2
• UniProt Synonym Gene Names: CHK2; RAD53
• UniProt Entry Name: CHK2_HUMAN

NCBI Description

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Chk2: a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; EC 2.7.11.1; CAMK group; RAD53 family

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: nucleoplasm; Golgi apparatus; PML body; chromosome, telomeric region

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; metal ion binding; ubiquitin protein ligase binding; protein kinase binding; ATP binding

Biological Process: transcription, DNA-dependent; protein stabilization; DNA damage induced protein phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; DNA damage response, signal transduction; protein amino acid phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; cellular protein catabolic process; cell division; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; response to DNA damage stimulus

Disease: Li-fraumeni Syndrome 2; Breast Cancer; Prostate Cancer; Osteogenic Sarcoma