Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-BRCA1 (Ser1423) antibody.)
Rabbit BRCA1 Polyclonal Antibody | anti-phospho-BRCA1 antibody
Anti-phospho-BRCA1 (Ser1423)
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity chromatography
Synonyms
BRCA1; Polyclonal Antibody; Anti-phospho-BRCA1 (Ser1423); anti-phospho-BRCA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the BRCA1 protein phosphorylated at serine 1423
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-BRCA1 antibody
Immunohistochemistry (IHC)
Application Notes
IHC: 1:50-1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1423
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-BRCA1 (Ser1423) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-BRCA1 (Ser1423) antibody.)
Related Product Information for anti-phospho-BRCA1 antibody
The breast cancer susceptibility protein BRCA1 is responsible for repairing DNA damage, controlling the cell cycle, transcriptional regulation, ubiquitination and maintaining genomic stability. BRCA1 interacts with several protein complexes through interactions with different adaptor molecules. For example, BRCA1 is a component of the RNA polymerase II holoenzyme (PolII)(1). Because of its role in maintaining DNA fidelity, mutations that affect the subcellular localization of BRCA1 can lead to cancer predisposition (2). BRCA1 is most commonly associated with breast cancer, but it is also implicated other cancers such as ovarian cancer. BRCA1 is phosphorylated at Ser988 after DNA damage and phosphorylated at Ser1423/1524 in response to radiation exposure (3,4).
Product Categories/Family for anti-phospho-BRCA1 antibody
References
1. Scully, R. et al: BRCA1 is a component of the RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5605-10.
2. Chen, Y. et al: Aberrant subcellular localization of BRCA1 in breast cancer. Science 1995 Nov 3;270(5237):789-91.
3. Lee J S. et al: hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response. Nature. 2000 Mar 9;404(6774):201-4.
4. Cortez D. et al: Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999 Nov 5;286(5442):1162-6.
2. Chen, Y. et al: Aberrant subcellular localization of BRCA1 in breast cancer. Science 1995 Nov 3;270(5237):789-91.
3. Lee J S. et al: hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response. Nature. 2000 Mar 9;404(6774):201-4.
4. Cortez D. et al: Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999 Nov 5;286(5442):1162-6.
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Product Notes
The phospho-BRCA1 (Catalog #AAA515882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-BRCA1 (Ser1423) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRCA1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). IHC: 1:50-1:100. Researchers should empirically determine the suitability of the phospho-BRCA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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