p33ING1 recombinant protein

p33ING1 recombinant protein

Cat. Num. AAA515174

• Gene Names: ING1; p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• Applications: Western Blot
• Synonyms: p33ING1; p33ING1 recombinant protein; ING1; p33; p47; p24ING1c; p33ING1b; p47ING1a

• Host: Sf9 insect cells
• Form/Format: 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 2887

• Applicable Applications for p33ING1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant full-length human p33ING1 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for p33ING1 recombinant protein

Recombinant full-length human p33ING1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Scientific Background: p33ING1 displays characteristics of a tumour suppressor protein and can induce cell growth arrest and apoptosis. p33ING1 is a nuclear protein and acute expression of p33 ING1 inhibits cell growth. p33ING1 physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Mutation of p33ING1 gene occurs in neuroblastoma cells and reduced expression is observed in some breast cancer cell lines (1). It has been proposed that p33ING1 can act as a potent growth regulator in normal and in established cells and plays a role as a candidate tumour suppressor gene whose inactivation may contribute to the development of cancers (2).

Product Categories/Family for p33ING1 recombinant protein

Cell Cycle Proteins; Signaling Proteins - Cell Cycle Proteins

References

1. Garkavtsev, I. Et al: Suppression of the novel growth inhibitor p33ING1 promotes neoplastic transformation. Nat Genet, 1999; 23(3):373.
2. Goeman, F. Et al: Growth Inhibition by the Tumor Suppressor p33ING1 in Immortalized and Primary Cells: Involvement of Two Silencing Domains and Effect of Ras.

NCBI and Uniprot Product Information

• NCBI GI #: 392050742
• NCBI GeneID: 3621
• NCBI Accession #: NP_937862.1
• NCBI GenBank Nucleotide #: NM_198219
• UniProt Accession #: Q9UK53; O00532; O43658; Q53ZR3; Q5T9G8; Q5T9G9; Q5T9H0; Q5T9H1; Q9H007; Q9HD98; Q9HD99; Q9NS83
• Molecular Weight: ~38 kDa
• NCBI Official Full Name: Homo sapiens inhibitor of growth family, member 1 (ING1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: inhibitor of growth family, member 1
• NCBI Official Symbol: ING1
• NCBI Official Synonym Symbols: p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• NCBI Protein Information: inhibitor of growth protein 1; growth inhibitor ING1; tumor suppressor ING1; growth inhibitory protein ING1
• UniProt Protein Name: Inhibitor of growth protein 1
• UniProt Gene Name: ING1
• UniProt Entry Name: ING1_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. Ref.13

Subunit structure: Interacts with H3K4me3 and to a lesser extent with H3K4me2. Interacts with TP53. Ref.13 Ref.14

Subcellular location: Nucleus

Probable.

Tissue specificity: Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines. Ref.3

Domain: The PHD-type zinc finger mediates the binding to H3K4me3. Ref.14The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P)

By similarity. Ref.14

Involvement in disease: Squamous cell carcinoma of the head and neck (HNSCC) [MIM:275355]: A non-melanoma skin cancer affecting the head and neck. The hallmark of cutaneous SCC is malignant transformation of normal epidermal keratinocytes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5

Sequence similarities: Belongs to the ING family.Contains 1 PHD-type zinc finger.

Sequence caution: The sequence AAB60879.1 differs from that shown. Reason: Frameshift at position 149. The sequence AAG02579.1 differs from that shown. Reason: Erroneous gene model prediction.