Rabbit anti-Rat NMDA NR2B Subunit (Ser1480) Polyclonal Antibody | anti-GRIN2B antibody

Anti-Phospho-Ser1480 NMDA Receptor NR2B Subunit

Cat. Num. AAA502088

• Gene Names: Grin2b; GluN2B
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: NMDA NR2B Subunit (Ser1480); Polyclonal Antibody; Anti-Phospho-Ser1480 NMDA Receptor NR2B Subunit; anti-GRIN2B antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Ser1480. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1482

• Applicable Applications for anti-GRIN2B antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser1480 of the NR2B subunit of the rat NMDA receptor.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates, and Xenopus
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-GRIN2B antibody

Affinity purified rabbit polyclonal antibody

References

• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843.
• Takasu, MA, Dalva, MB, Zigmond, RE, Greenberg, ME (2002) Modulation of NMDA Receptor -Dependent Calcium Influx and Gene Expression Through EphB Receptors. Science 295:491-495.
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.

NCBI and Uniprot Product Information

• NCBI GI #: 6980984
• NCBI GeneID: 24410
• NCBI Accession #: NP_036706.1
• NCBI GenBank Nucleotide #: NM_012574.1
• UniProt Accession #: Q00960; Q62684
• Molecular Weight: 180
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• NCBI Official Symbol: Grin2b
• NCBI Official Synonym Symbols: GluN2B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2b
• UniProt Synonym Gene Names: GluN2B; NMDAR2B; NR2B
• UniProt Entry Name: NMDE2_RAT

NCBI Description

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death

By similarity.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1

By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity

By similarity.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]