Western Blot (WB)
(Western Blot using anti-CD171 antibody L1-14.10 (MBS488699). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (MBS488698) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Human CD171 Monoclonal Antibody | anti-CD171 antibody
Anti-CD171 [L1-14.10]
Western Blot (WB)
(Western Blot using anti-CD171 antibody L1-14.10 (MBS488699). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (MBS488698) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD171 antibody L1-14.10 (MBS488699). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (MBS488698) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of HeLa cells using anti-CD171 (MBS488699) L1-14.10 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of L1-14.10 (MBS488698) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of HeLa cells using anti-CD171 (MBS488699) L1-14.10 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of L1-14.10 (MBS488698) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Western Blot (WB)
(Western Blot using anti-CD171 antibody L1-14.10 (MBS488699). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (MBS488698) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD171 antibody L1-14.10 (MBS488699). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (MBS488698) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD171 antibody L1-14.10 (MBS488699). Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (MBS488140; isotype control, black line) or the mouse IgG1 version of L1-14.10 (MBS488698, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD171 antibody L1-14.10 (MBS488699). Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (MBS488140; isotype control, black line) or the mouse IgG1 version of L1-14.10 (MBS488698, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Note on publication: Describes the original generation of this antibody, and its use in immunohistochemical analysis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
Uniprot Description
NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: presynaptic membrane; cell surface; focal adhesion; plasma membrane; integral to membrane; terminal button; external side of plasma membrane
Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding
Biological Process: nervous system development; axon guidance; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; cell adhesion; blood coagulation; homophilic cell adhesion; leukocyte migration
Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome
Research Articles on CD171
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Product Notes
The CD171 l1cam (Catalog #AAA488699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD171 [L1-14.10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD171 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). The specificity of this antibody has been confirmed in flow cytometric (Schafer et al, 2012; Huszar et al, 2006) and Western blot analysis (Huszar et al, 2006; Wolterink et al, 2010). This antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma cells, to efficiently and sustainedly block extracellular signal-regulated kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used in immunohistochemical staining for L1 expression, using paraffin-embedded human ovarian carcinoma tissue sections (Wolterink et al, 2010; Knogler et al, 2007) and a panel of human tumour tissue sections, including benign and malignant tumours of the female genital tract (Huszar et al, 2006), and renal cell carcinoma, schwannoma, gastric conventional GIST, pancreatic neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody, when administered to SCID mice in combination with cytostatic drugs, efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip tumors (Schafer et al, 2012). Researchers should empirically determine the suitability of the CD171 l1cam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD171, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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