Human Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) RTU ELISA Kit | HNRPK rtu elisa kit

Human Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) ELISA Kit

Cat. Num. AAA459887

• Gene Names: HNRNPK; AUKS; CSBP; TUNP; HNRPK
• Reactivity: Human
• Synonyms: Heterogeneous Nuclear Ribonucleoprotein K (HNRPK); Human Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) ELISA Kit; hnRNP-K; CSBP; HNRNPK; TUNP; Transformation Upregulated Nuclear Protein; HNRPK rtu elisa kit

• Reactivity: Human
• Sequence Length: 439

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.107ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRPK rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNRPK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HNRPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HNRPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNRPK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 970598247
• NCBI GeneID: 3190
• NCBI Accession #: NP_001305115.1
• NCBI GenBank Nucleotide #: NM_001318186.1
• UniProt Accession #: P61978; Q07244; Q15671; Q59F98; Q5T6W4; Q60577; Q6IBN1; Q922Y7; Q96J62
• Molecular Weight: 48,562 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein K isoform c
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein K
• NCBI Official Symbol: HNRNPK
• NCBI Official Synonym Symbols: AUKS; CSBP; TUNP; HNRPK
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein K
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein K
• UniProt Gene Name: HNRNPK
• UniProt Synonym Gene Names: HNRPK; hnRNP K; TUNP

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.