Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 cells using anti-c-erbB-2 FWP51 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of FWP51 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Mouse anti-Human c-erbB-2 Monoclonal Antibody | anti-c-erbB-2 antibody
Anti-c-erbB-2 [FWP51], Mouse IgG1, Kappa
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified
Synonyms
c-erbB-2; Monoclonal Antibody; Anti-c-erbB-2 [FWP51]; Mouse IgG1; Kappa; CD340; Receptor tyrosine-protein kinase erbB-2; Neu; HER2; MLN19; Metastatic lymph node gene 19 protein; p185erbB2; anti-c-erbB-2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
FWP51
Specificity
FWP51 binds to the extracellular domain of c-erbB-2.
Purity/Purification
Purified
Form/Format
PBS with 0.02% Proclin 300
Applicable Applications for anti-c-erbB-2 antibody
Fusion, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993).
Antibody Type
Recombinant Antibody
Immunogen
20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 cells using anti-c-erbB-2 FWP51 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of FWP51 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 cells using anti-c-erbB-2 FWP51 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of FWP51 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-c-erbB-2 antibody
Describes the original production of the antibody and its characterisation through immunofluorescence and immunoprecipitation.
Recombinant monoclonal antibody to c-erbB-2. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma FWP51.
Recombinant monoclonal antibody to c-erbB-2. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma FWP51.
Product Categories/Family for anti-c-erbB-2 antibody
References
Harwerth et al, 1992.; J Biol Chem. 1992 Jul 25;267(21):15160-7.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The c-erbB-2 (Catalog #AAA488526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-c-erbB-2 [FWP51], Mouse IgG1, Kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB-2 can be used in a range of immunoassay formats including, but not limited to, Fusion, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993). Researchers should empirically determine the suitability of the c-erbB-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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