Mouse anti-Human PRK2 Monoclonal Antibody | anti-PRK2 antibody

Anti-PRK2 Mouse mAb

Cat. Num. AAA476200

• Gene Names: PKN2; PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
• Reactivity: Human
• Applications: Western Blot
• Synonyms: PRK2; Monoclonal Antibody; Anti-PRK2 Mouse mAb; Cardiolipin activated protein kinase Pak2; MGC150606; MGC71074; PAK 2; PAK2; PKN gamma; PKN2; PKN2_HUMAN; PRKCL2; PRO2042; Protein kinase C like 2; Protein kinase C related kinase 2; Protein kinase C-like 2; Protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; anti-PRK2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4C2-B2-A0
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 936

• Applicable Applications for anti-PRK2 antibody: Western Blot (WB)
• Immunogen: Purified recombinant fragment of human PRK2 expressed in E Coli.
• Sequence Similarities: Contains 3 REM (Hr1) repeats.
• Subunit Structure: Interacts (via the REM repeats) with RHOA (GTP-bound form preferentially). Interacts (via the REM repeats) with RAC1 (GTP-bound form preferentially). Interacts with NCK1 (via SH3 domains). Interacts with CD44 (By similarity). Interacts with RHOA (GTP-bound form preferentially) and RAC1 (GTP-bound form preferentially); the interactions induce its autophosphorylation. Interacts (via C-terminal kinase domain) with PDPK1; the interaction stimulates PDPK1 kinase activity. Interacts with MAP3K2; the interaction activates PRK2 kinase activity in a MAP3K2-independent kinase activity. Interacts (via C-terminal domain) with AKT1; the interaction occurs with the C-terminal cleavage product of PRK2 in apoptotic cells. Interacts (via C-terminus) with PTPN13 (via PDZ 3 domain). Interacts with HCV NS5B (via N-terminal finger domain). Interacts with NCK1, NCK2 and RHOC.
• Subcellular Location: Cell junction

Western Blot (WB)

(Western blot detection of PRK2 in HUVEC, A549, Jurkat, K562 and Hela cell lysates using PRK2 mouse mAb (1:500 diluted).Predicted band size:140KDa.Observed band size:140KDa.)

Western Blot (WB)

(Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 mouse mAb with DAB staining.)

Related Product Information for anti-PRK2 antibody

Entrez Summary: Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
UniPort Summary Function: PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

NCBI and Uniprot Product Information

• NCBI GI #: 1002635082
• NCBI GeneID: 5586
• NCBI Accession #: NP_001307636.1
• NCBI GenBank Nucleotide #: NM_001320707.1
• UniProt Accession #: Q16513; Q08AF4; Q9H1W4; B4DQ21; B4DTP5; B4DVG1; D3DT24
• Molecular Weight: 140kDa
• NCBI Official Full Name: serine/threonine-protein kinase N2 isoform 2
• NCBI Official Synonym Full Names: protein kinase N2
• NCBI Official Symbol: PKN2
• NCBI Official Synonym Symbols: PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
• NCBI Protein Information: serine/threonine-protein kinase N2
• UniProt Protein Name: Serine/threonine-protein kinase N2
• Protein Family: Pollen receptor-like kinase
• UniProt Gene Name: PKN2
• UniProt Synonym Gene Names: PRK2; PRKCL2

Uniprot Description

PKN2: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase and Rho/Rac effector protein. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcriptional activation. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. Kinase activity is activated upon binding to GTP-bound Rhoa/Rac1 GTPases. Activated by limited proteolysis with trypsin, and withn caspase-3 (CASP3) during apoptosis. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm. Five isoforms of the human protein are produced by alternative splicing.

Protein type: AGC group; EC 2.7.11.13; Kinase, protein; PKN family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: apical junction complex; cell junction; cell-cell adherens junction; centrosome; cleavage furrow; cytoplasm; cytosol; intermediate filament cytoskeleton; lamellipodium; midbody; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: histone deacetylase binding; kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: apical junction assembly; peptidyl-serine phosphorylation; positive regulation of cytokinesis; positive regulation of mitotic cell cycle; positive regulation of viral genome replication; protein amino acid phosphorylation; signal transduction