Mouse ALK1 ELISA Kit | ACVRL1 elisa kit

Mouse ALK1 ELISA Kit

Cat. Num. AAA8248417

• Gene Names: Acvrl1; Alk1; Acvrlk1; AI115505; AI427544
• Reactivity: Mouse
• Synonyms: ALK1; Mouse ALK1 ELISA Kit; ACVRLK1; Serine/threonine-protein kinase receptor R3; SKR3; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I; ACVRL1 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse ALK1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse ALK1 proteins within the range of 46.9 pg/ml - 3000 pg/ml.
• Sequence Length: 502

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACVRL1 elisa kit

Principle of the Assay: The Mouse ALK1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ALK1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse ALK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ALK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse ALK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ALK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

NCBI and Uniprot Product Information

• NCBI GI #: 471015970
• NCBI GeneID: 11482
• NCBI Accession #: NP_001264186.1
• NCBI GenBank Nucleotide #: NM_001277257.1
• UniProt Accession #: Q61288; Q61289; Q91YR0
• Molecular Weight: 56,519 Da
• NCBI Official Full Name: serine/threonine-protein kinase receptor R3 isoform a
• NCBI Official Synonym Full Names: activin A receptor, type II-like 1
• NCBI Official Symbol: Acvrl1
• NCBI Official Synonym Symbols: Alk1; Acvrlk1; AI115505; AI427544
• NCBI Protein Information: serine/threonine-protein kinase receptor R3
• UniProt Protein Name: Serine/threonine-protein kinase receptor R3
• Protein Family: Serine/threonine-protein kinase receptor
• UniProt Gene Name: Acvrl1
• UniProt Synonym Gene Names: Acvrlk1; Alk-1; SKR3; ALK-1; TSR-I
• UniProt Entry Name: ACVL1_MOUSE

Uniprot Description

ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type1 subfamily

Cellular Component: cell soma; cell surface; dendrite; integral to plasma membrane; plasma membrane

Molecular Function: activin binding; activin receptor activity, type I; ATP binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type I

Biological Process: angiogenesis; blood circulation; blood vessel morphogenesis; blood vessel remodeling; BMP signaling pathway; endothelial cell activation; in utero embryonic development; lymphangiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of cell adhesion; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; negative regulation of focal adhesion formation; positive regulation of angiogenesis; positive regulation of BMP signaling pathway; positive regulation of endothelial cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; regulation of blood pressure; regulation of transcription, DNA-dependent; signal transduction; transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells