LIMK1 lysate
LIMK1 (Activated) Lysate
Applications
Western Blot
Synonyms
LIMK1; LIMK1 (Activated) Lysate; LIMK1 lysate
Form/Format
Supplied at a concentration of 2 ng/ul in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Sequence Length
209
Applicable Applications for LIMK1 lysate
Western Blot (WB)
Application Notes
WB: 10 mul/lane
Lysate Preparation
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rhofamily GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr -505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin.
An activated His-tagged mouse LIMK1 contains Thr-508 phosphorylation. The phosphorylated kinase is detected by anti-LIMK1 (N-terminus) and anti-LIMK1 (Thr-508). This activated kinase can be dephosphorylated at Thr-508 by treating with l-phosphatase for 30 min. at 37 degree C.
An activated His-tagged mouse LIMK1 contains Thr-508 phosphorylation. The phosphorylated kinase is detected by anti-LIMK1 (N-terminus) and anti-LIMK1 (Thr-508). This activated kinase can be dephosphorylated at Thr-508 by treating with l-phosphatase for 30 min. at 37 degree C.
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LIMK1 (Catalog #AAA474115) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LIMK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10 mul/lane. Researchers should empirically determine the suitability of the LIMK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMK1, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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