Western Blot (WB)
(Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-betaTubulin (a.a. 168-177) (lanes 1 & 2), anti-beta-Tubulin (Ser-172) (lanes 3 & 4), and anti-beta-Tubulin (TM1541) (lanes 5 & 6).)
Tubulin Lysate
Tubulin (Bovine Brain) Lysate
Applications
Western Blot
Synonyms
Tubulin; Tubulin (Bovine Brain) Lysate; Tubulin lysate
Form/Format
Supplied at a concentration of 20 ng/ul in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Sequence Length
423
Applicable Applications for Tubulin lysate
Western Blot (WB)
Application Notes
WB: 10 mul/lane
Lysate Preparation
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/beta-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability.
For preparation of tubulin lysate, bovine brain tubulin was purified and quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.
For preparation of tubulin lysate, bovine brain tubulin was purified and quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-betaTubulin (a.a. 168-177) (lanes 1 & 2), anti-beta-Tubulin (Ser-172) (lanes 3 & 4), and anti-beta-Tubulin (TM1541) (lanes 5 & 6).)
Western Blot (WB)
(Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-betaTubulin (a.a. 168-177) (lanes 1 & 2), anti-beta-Tubulin (Ser-172) (lanes 3 & 4), and anti-beta-Tubulin (TM1541) (lanes 5 & 6).)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Tubulin (Catalog #AAA474146) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10 mul/lane. Researchers should empirically determine the suitability of the Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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