Unphosphorylated WAVE (Tyr-150) Peptide

Synonyms

Unphosphorylated WAVE (Tyr-150) Peptide; Unphosphorylated WAVE (Tyr-150) Peptide peptide

Ordering
Cat. Num. AAA474067 For Research Use Only!

Specificity

This peptide can be used as an unphosphorylated control peptide for WAVE (Tyr-150) antibody (WP1821). It is the unphosphorylated version of phospho-WAVE (Tyr-150) peptide (WX1825), and it does not block WP1821 reactivity in Western blot, demonstrating the phospho-specificity of WP1821.

Form/Format

Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.

Sequence

Peptide Sequence: Unphosphorylated WAVE (Tyr-150) synthetic peptide corresponds to amino acid residues surrounding the Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and to similar regions in the conserved site in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).

Application Notes

Blocking: 1:1,000
ELISA: 50 ng/well

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Related Product Information for Unphosphorylated WAVE (Tyr-150) Peptide peptide

The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actinmediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

References

• Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.

Similar Products

Product Notes

The Unphosphorylated WAVE (Tyr-150) Peptide (Catalog #AAA474067) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Blocking: 1:1,000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the Unphosphorylated WAVE (Tyr-150) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Unphosphor ylated WAVE (Tyr-150) synthetic peptide correspond s to amino acid residues surroundin g the Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and to similar regions in the conserved site in WAVE1 (Tyr-151) and WAVE3 (Tyr-151). It is sometimes possible for the material contained within the vial of "Unphosphorylated WAVE (Tyr-150) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Unphosphorylated WAVE (Tyr-150) Peptide