Testing Data

phospho-c-Abl (Tyr-412) Peptide

Synonyms

phospho-c-Abl (Tyr-412) Peptide; phospho-c-Abl (Tyr-412) Peptide peptide

Ordering
Cat. Num. AAA474027 For Research Use Only!

Specificity

The peptide is specifically recognized by anti-c-Abl (Tyr-412) phospho-specific antibody (AP1271) in ELISA, and has been used to block the reactivity of AP1271 during Western blot. In addition, the peptide is recommended for use in blocking AP1271 reactivity in immunocytochemistry.

Form/Format

Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.

Sequence

Peptide Sequence: Phospho-c-Abl (Tyr-412) synthetic peptide corresponds to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.

Application Notes

Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Testing Data

Related Product Information for phospho-c-Abl (Tyr-412) Peptide peptide

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways.

References

• Brasher, B.B. et al. (2000) J. Biol. Chem. 275:35631.
• Pluk, H. et al. (2002) Cell 108:247.
• Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9:179.

NCBI and Uniprot Product Information

NCBI GI #

179750

NCBI Official Full Name

c-abl

Similar Products

Product Notes

The phospho-c-Abl (Tyr-412) Peptide (Catalog #AAA474027) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1 ug/ml ELISA: 10-100 ng/well. Researchers should empirically determine the suitability of the phospho-c-Abl (Tyr-412) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-c- Abl (Tyr-412) synthetic peptide correspond s to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein. It is sometimes possible for the material contained within the vial of "phospho-c-Abl (Tyr-412) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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phospho-c-Abl (Tyr-412) Peptide