Testing Data
phospho-SHP1 (Tyr-536) Peptide | shp1 peptide
phospho-SHP1 (Tyr-536) Blocking Peptide
Synonyms
phospho-SHP1 (Tyr-536) Peptide; phospho-SHP1 (Tyr-536) Blocking Peptide; shp1 peptide
Specificity
The peptide is specifically recognized by anti-SHP1 (Tyr-536) phospho-specific antibody (SP1571) in ELISA, and has been shown to block the reactivity of SP1571 during Western blot. In addition, the peptide is recommended for use in blocking SP1571 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-SHP1 (Tyr-536) synthetic peptide corresponds to amino acids around tyrosine 536 of human SHP1. The sequence is highly conserved in rat and mouse SHP1.
Sequence Length
74
Application Notes
Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well
ELISA: 10-100 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for shp1 peptide
SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKC-alpha and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.
References
• Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
• Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
• Zhang, J. et al. (2000) Semin. Immunol. 12:361.
• Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
• Zhang, J. et al. (2000) Semin. Immunol. 12:361.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
8,175 Da
NCBI Official Full Name
SHP1
NCBI Official Symbol
shp1
UniProt Protein Name
SHP1
UniProt Gene Name
shp1
UniProt Entry Name
O93799_LENED
Similar Products
Product Notes
The shp1 shp1 (Catalog #AAA474056) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1 ug/ml ELISA: 10-100 ng/well. Researchers should empirically determine the suitability of the shp1 shp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-SH P1 (Tyr-536) synthetic peptide correspond s to amino acids around tyrosine 536 of human SHP1. The sequence is highly conserved in rat and mouse SHP1. It is sometimes possible for the material contained within the vial of "phospho-SHP1 (Tyr-536) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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